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515 results sorted by
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?
No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...
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They are embryonic. The rats are E18 or E19 and the mice are E14 or E15.
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l
?
We do not recommend reusing the conductive polymer cuvettes as performance decreases when cuvettes are used more than once.
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The rats are Sprague-Dawley.
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Cells might have been disturbed or ran dry during pipetting steps. Please perform liquid removal and additions well-by-well. Avoid leaving neurons without any liquid coverage (medium or Nucleofector® Solution). To keep a small liquid film on the...
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S
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p
?
This depends on the cell type you are working with. In our Optimized Protocols the cell numbers range from 2.5 x 10^4 cells (mouse DC) up to 1x 10^6 cells (human T cells). Detailed information is provided in our 4D-Nucleofector® Optimized Protocols.
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?
The cells can remain viable in culture for about 28 days.
D
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®
?
No, each well can be addressed separately. You may apply up to 16 different programs to one 16-well Nucleocuvette® Strip.
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?
We would recommend to prepare the culture plate as such that cells are only plated into those wells that should be transfected and leave further wells empty.After withdrawing the dipping electrode array from the plate you can wipe off residual liquid...
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f
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p
l
a
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g
?
No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware
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