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Do your Clonetics Rat and Mouse Astrocytes proliferate?

Yes. The cells do expand in culture.

What age are the mice and rats that your Clonetics Mouse and Rat Astrocytes are isolated from?

They are embryonic. The rats are E18 or E19 and the mice are E14 or E15.

What primary human diseased airway cells does Lonza offer?

Bronchial Epithelial Cells, Lung Fibroblasts, and Bronchial Smooth Muscle Cells from asthmatic and COPD donors and if available from donors with Cystic fibrosis. An overview of the diseased cells can be found at:...

How is the disease state for Lonzas diseased airway cells determined?

The tissue received for all of Lonza’s diseased airway cells are obtained from donors which were diagnosed by an accredited physician prior to death. Frequently, Lonza will obtain information on medications taken related to the disease which will...

How does Lonza test for quality of the diseased airway cells?

Diseased Bronchial Epithelial Cells are characterized by morphology through serial passage and some are tested for differentiation using B-ALI™ Differentiation protocols, these are tested for TEER levels, ciliogenesis, and mucin production. Diseased...

Do your Clonetics Rat and Mouse Astrocytes require an extracellular matrix for plating?

No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware

What medium do you recommend for the diseased airway cells?

The recommended medium for diseased cells is the same as normal cells. BEGM™ for Diseased Bronchial Epithelial Cells, some lots are guaranteed in B-ALI™ Media. SmGM™-2 Medium is recommended for Diseased Bronchial Smooth Muscle Cells. FGM™-2 Medium...

What age are the rats that your Clonetics Rat DRGs are isolated from?

They R-DRG-505 are isolated from neonatal, about 2-3 days old. The R-eDRG-515 are isolated from embryonic rats (E14-15).