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What donor characteristics are available for the diseased airway cells?

Most Clonetics™ Primary Cells have donor information on the Certificate of Analysis (CoA). This information usually includes age, sex, and race. Sometimes additional information may be obtained by contacting Lonza Scientific Support - if this...

Do I need to purchase a special chamber to use Reliant™ and Latitude™ Precast Gels?

Reliant™ and Latitude™ Precast Agarose Gels are designed to run in standard horizontal electrophoresis chambers.As long as there is room on the chamber platform for the gel, the chamber should be suitable. Measure the chamber platform and check...

Are there any contaminating glial cells in your Clonetics™ rat or mouse embryonic neuronal cells?

There will be a small amount of glial cells in the culture, these are not considered a contaminating cell type. The glial cells are necessary to help the neurons form their axons and dendrites, synchronize the activity of the axons, and remove...

Are the materials in the FlashGel™ Cassette hazardous?

The stain in the FlashGel™ Cassette is present at such low levels that it is not considered hazardous according to OSHA and EU hazard criteria. A copy of the MSDS is available online. However, as the stain in the cassette is a potential mutagen, wear...

Do your Clonetics™ Rat and Mouse Astrocytes proliferate?

Yes. The cells do expand in culture.

Do I need to use a gelatin, fibronectin, collagen, or Matrigel™ coating when culturing my Clonetics™ Cells?

Most Clonetics™ Cells do not require an extracellular matrix when grown on tissue culture treated plastic – flasks, dishes, well plates (with the exception of hepatocytes, and the rat/mouse neuronal and dorsal root ganglion neurons, rat cardiac...

What age are the mice and rats that your Clonetics™ Mouse and Rat Astrocytes are isolated from?

They are embryonic. The rats are E18 or E19 and the mice are E14 or E15.

Your instructions for culturing of Clonetics™ Cells do not mention centrifuging the cells upon thawing. Won't the DMSO kill the cells?

We have seen on multiple occasions that the effects of centrifugation can be significantly more damaging than the effects of residual DMSO on primary cells. As such, unless specifically noted in the recommended culture protocol, instead of...

I stained my gel with SYBR Green and recovered my band of interest. However, my DNA is still green. Can I remove the SYBR stain?

Yes. The SYBR stains can easily be removed from nucleic acids by ethanol precipitation. Isopropyl alcohol precipitation is somewhat less effective at removing the dye. Butyl alcohol extraction, chloroform extraction and phenol extraction do not...

What species of rat are the Clonetics™ Rat Astrocytes isolated from?

The rats are Sprague-Dawley.

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