We have seen on multiple occasions that the effects of centrifugation can be significantly more damaging than the effects of residual DMSO on primary cells.

As such, unless specifically noted in the recommended culture protocol, instead of centrifuging the cells, we recommend plating the cells directly at our recommended plating density for that cell type. When plated at this density, the residual DMSO will not be sufficient to affect the cells and the cells will not be harmed by the effects of a centrifugation step. When plating the cells directly, we recommend changing the media after the first 24 hours (to remove the residual DMSO), then change the media every 48 hours after that point with 1 ml of media per every 5 scm of growth area (or 15 ml of media per every T-75 flask).

(The only exception are the CC-2931 InEpC)