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How much DNA should I load per well of an agarose gel?

The amount to load per well is variable. What is most important is how much DNA there is in the bands you wish to resolve. The least amount of DNA that can be consistently detected with ethidium bromide is about 10 ng. The most DNA you can have in a...

What additional donor information is available for Lonza’s diseased airway cells?

In addition to the standardly available donor information (age, gender, race), Lonza can provide the following additional information for most lots of our diseased airway cells: - smoking history (including volume and duration), - any medications...

Why is the morphology of Lonza’s lung microvascular endothelial cells (HMVEC-L) CC-2527 different from most other endothelial cells?

While most endothelial cells will have a “paving stone” type morphology, Lonza’s lung microvascular endothelial cells (HMVEC-L) will generally appear more stretched and elongated, especially at low confluence. This is a normal morphological...

At what voltage should I run an agarose gel?

We recommend running agarose gels at 4–10 volts/cm (cm is determined by measuring the interelectrode distance, not the gel length) under normal horizontal electrophoretic conditions.If the voltage is too high, band streaking, especially for DNA...

Will Lonza’s diseased airway cells retain their diseased phenotype throughout culturing?

With limited exceptions, Lonza does not test the diseased cells as part of our standard Quality Control testing in a manner which would validate the disease diagnosis. All of Lonza’s diseased airway cells are cultured using the same medium and...

Where are Lonza’s Normal Human Neural Progenitor (NHNP) and Normal Human Astrocytes (NHA) cells isolated from?

Lonza’s Normal Human Neural Progenitor (NHNP) and Normal Human Astrocytes (NHA) cells are primarily isolated from the cerebral cortex (or forebrain from cerebral hemisphere) from fragmented fetal brain tissue (16-20 weeks old).

What is the difference between Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium?

Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s BEGM™ medium just prior to differentiation using the...

What is the difference between NuSieve™ 3:1 and NuSieve™ GTG™ Agaroses?

NuSieve™ 3:1 Agarose is a standard melting temperature agarose. The resolution range for NuSieve™ 3:1 Agarose is 50 bp–1000 bp. NuSieve™ 3:1 Agarose is designed for analytical electrophoresis; its high gel strength also makes it ideal for use in...

What is a GTG™ Agarose?

GTG™ stands for Genetic Technology Grade™. GTG™ Grade Agarose is recommended for preparative DNA electrophoresis, or when further enzymatic manipulation of DNA is required. These agaroses are extensively tested to ensure maximum compatibility with...

What is the difference between Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium?

Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s SAGM™ medium just prior to differentiation using the...
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