Q:

Do I need to shake the plate after adding the Cell Lysis Reagent during the ViaLightâ„¢ Assay?

A:
No. Shaking the plate during this step can cause frothing which will deflect the light signal away from the detection unit, reducing the number of RLUs observed and producing an artificially low result.
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Categories:
Bioassay
Research Areas:
Uncategorized
Toxicology