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515 results sorted by
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A
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Yes. We do test the Solutions with a real time RT-PCR based assay. This assay basically measures the quantity of an RNA template after incubation in our solution. In case there is RNAse contamination the template would be digested which would be...
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?
No, the programs C-20 and C-020 are identical regarding their electrical parameters. The letter minus a two-digit number is the nomenclature of the Nucleofector™ I. Since we have launched the Nucleofector™ II we changed it to a letter minus a three...
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Please use the buffer that is recommended by your siRNA manufacturer.
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7
A
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m
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p
h
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?
The results obtained using 7AAD staining are much clearer than those obtained using propidium iodide (PI). Using flow cytrometry stained and unstained, transfected and non-transfected populations can be separated much more easily.
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We use a stock concentration of 100 µg/ml in PBS. This is diluted 1:300 – 1:400 into the sample.
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H
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K
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?
GFP expression has been observed for up to one week as measured by light and fluorescence microscopy.
D
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f
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c
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l
l
s
?
Insect cells are cultivated at lower temperatures than E.coli or mammalian cells (S2 cells at 24°C, Sf9 cells at 28°C).Therefore they need a dedicated incubator with appropriate temperature.
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p
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s
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o
n
i
n
m
a
m
m
a
l
i
a
n
c
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l
l
s
?
Vectors with a CMV promoter typically work fine for transient protein expression in mammalian cells, like HEK293, CHO or NSO. For insect cells, like Sf9 or Sf21, vectors with insect promoters are required. In HEK293E (EBNA) cells, the use of...
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N
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®
E
x
p
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r
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m
e
n
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s
?
The optimal incubation time ranges from 2 to 6 days depending on various factors like the stability of the protein in the medium, potential toxicity of the protein, cell density and stability of the plasmid in the nucleus. To check the timepoint for...
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N
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T
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n
?
For many cell lines in suspension, especially sCHO, conventional transfection methods are very limited in efficiency. For protein production, suspension cells are favored over adherent cells because they are much easier to cultivate. Unlike...
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