Q:

When would be the optimal time to perform experiments post osteogenic differentiation for human mesenchymal stem cells?

A:
Once the cells have differentiated and started laying down bone matrix, we recommend assaying the cells immediately. If cells begin to delaminate (peel of the culture plate in layers), this would indicate that the cells are embedded in the calcium matrix and should be assayed immediately. Bone mineralization can also be visualized without delamination by observing (microscopically) bone nodules above the cell monolayer.
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Categories:
Primary Cells and Media
Research Areas:
Stem Cells
Regenerative medicine