Q:

Are the human mesenchymal stem cells more prone to become differentiated down one lineage over the other?

A:
MSCs are prone to differentiate down an adipogenic liniage and some may accumulate lipids in the growth media or the differentiation kits other than the adipogenic differentiation media. MSCs are especially prone to spontaneous adipogenic differentiation if allowed to reach confluence. As such, to maintain cells as undifferentiated cells, it is critical to subculture cells when they are subconfluent.
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Categories:
Primary Cells and Media
Research Areas:
Stem Cells
Basic Research
Regenerative medicine