Data Type
Cell Information
(857)
Citations
(4878)
FAQ
(532)
Culture Media
(82)
Category
Primary Cells and Media
(214)
Transfection
(168)
Laboratory Instrumentation
(70)
Endotoxin Detection
(55)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(31)
Serum-free and Speciality Media
(15)
Classical Media and Reagents
(9)
Cell Lines and Primary Cancer Cells
(7)
3D Cell Culture
(6)
Uncategorized
(4)
Cell Services
(3)
Bioprocess Containers
(2)
Live Cell Imaging
(2)
Informatics for QC Microbiology
(1)
Protozoa
(1)
+ Show All
Research Area
Uncategorized
(125)
Basic Research
(102)
Cancer Research/Cell Biology
(68)
Immunotherapy / Hematology
(68)
Endotoxin Testing
(52)
Stem Cells
(41)
Cardiovascular
(27)
Neurobiology
(27)
Respiratory Research
(26)
Molecular Biology
(23)
Regenerative medicine
(20)
Gene Expression
(19)
Toxicology
(19)
Dermatology/Tissue Engineering
(12)
Drug Discovery
(7)
Gastroenterology
(1)
Parasitology
(1)
+ Show All
532 results sorted by
relevance
alphabetical
newest first
oldest first
H
o
w
m
a
n
y
c
o
r
t
i
c
a
l
n
e
u
r
o
n
s
c
a
n
I
e
x
p
e
c
t
f
r
o
m
a
s
i
n
g
l
e
p
u
p
?
According to QBM Cell Sciences, you can expect 12 million cortical neurons from an average E18-E19 rat pup. You can expect 4 million cortical neurons from an average mouse pup.
W
h
a
t
i
s
t
h
e
m
a
x
i
m
u
m
a
m
o
u
n
t
o
f
D
N
A
I
c
a
n
u
s
e
i
n
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
™
E
x
p
e
r
i
m
e
n
t
?
The overall amount of 5 µg should not be exceeded. Look in the cell specific Optimized Protocol (OP) for the recommended total DNA amount of the cell type of interest.
W
h
e
n
s
h
o
u
l
d
I
b
e
g
i
n
l
o
o
k
i
n
g
f
o
r
t
h
e
L
u
c
i
f
e
r
a
s
e
s
i
g
n
a
l
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
The time course of plasmid expression after Nucleofection™ might be different from the time course seen with other transfection methods. We recommend looking at different time points and start with the first time point as early as 4 to 6 hours post...
D
o
e
s
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
r
e
c
o
g
n
i
z
e
i
f
t
h
e
c
u
v
e
t
t
e
p
l
a
t
e
i
s
i
n
b
a
c
k
w
a
r
d
s
?
No, it does not recognize the orientation of the plate. Please make sure that well A1 is positioned in the upper left corner of the retainer.
W
h
a
t
s
h
o
u
l
d
I
p
a
y
a
t
t
e
n
t
i
o
n
t
o
w
h
e
n
t
r
a
n
s
f
e
r
r
i
n
g
m
y
r
e
p
o
r
t
e
r
g
e
n
e
s
e
t
u
p
f
r
o
m
a
c
e
l
l
l
i
n
e
t
o
a
p
r
i
m
a
r
y
c
e
l
l
?
In general the overall expression level differs between different cell types. Don't expect the same level of gene induction when working with primary cells. You might need to change to other DNA amounts or ratios.
D
o
a
n
y
o
f
t
h
e
t
h
r
e
e
i
n
s
t
r
u
m
e
n
t
s
u
s
e
d
i
n
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
S
y
s
t
e
m
"
g
o
t
o
s
l
e
e
p
"
o
r
p
o
w
e
r
o
f
f
a
u
t
o
m
a
t
i
c
a
l
l
y
?
The energy options of the laptop are set as such that only the screensaver is activated, the harddisk won't turn down and hibernation is disabled. One can change these settings via the system control. However this is not recommended because by...
W
h
a
t
i
s
t
h
e
m
o
l
e
c
u
l
a
r
w
e
i
g
h
t
o
f
y
o
u
r
c
o
n
t
r
o
l
p
l
a
s
m
i
d
p
m
a
x
G
F
P
™
?
The molecular weight is approximately 2.3x10e6 g/mol based on the average MW of a base pair being 660 g/mol.
W
h
a
t
i
s
t
h
e
m
o
l
e
c
u
l
a
r
w
e
i
g
h
t
o
f
t
h
e
G
F
P
p
r
o
t
e
i
n
e
x
p
r
e
s
s
e
d
b
y
y
o
u
r
c
o
n
t
r
o
l
p
m
a
x
G
F
P
™
p
l
a
s
m
i
d
?
The molecular weight of the protein is about 27kDa.
W
h
e
r
e
c
a
n
I
f
i
n
d
w
h
a
t
t
h
e
e
r
r
o
r
c
o
d
e
s
o
n
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
o
r
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
m
e
a
n
?
In the back of the respective manuals, there is a chart giving a brief description of the error codes. If more information is needed or an error code is recurrent, it is best to contact Scientific Support for advice to determine if there is a...
A
c
c
o
r
d
i
n
g
t
o
w
h
i
c
h
s
t
a
n
d
a
r
d
i
s
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
d
e
v
i
c
e
c
e
r
t
i
f
i
e
d
?
The Nucleofector II was tested by TÜV Rheinland product safety GmbH and found to be in compliance with the following standards: IEC 61010-1:2001 (2nd edition) and EN 61010-1:2001 (2nd edition). TÜV Rheinland of North America Inc. tested the...
PREVIOUS
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
PAGE
12
NEXT