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How many cortical neurons can I expect from a single pup?

According to QBM Cell Sciences, you can expect 12 million cortical neurons from an average E18-E19 rat pup. You can expect 4 million cortical neurons from an average mouse pup.

What is the maximum amount of DNA I can use in my Nucleofection Experiment?

The overall amount of 5 µg should not be exceeded. Look in the cell specific Optimized Protocol (OP) for the recommended total DNA amount of the cell type of interest.

When should I begin looking for the Luciferase signal after Nucleofection?

The time course of plasmid expression after Nucleofection™ might be different from the time course seen with other transfection methods. We recommend looking at different time points and start with the first time point as early as 4 to 6 hours post...

Does the 96-well Shuttle recognize if the cuvette plate is in backwards?

No, it does not recognize the orientation of the plate. Please make sure that well A1 is positioned in the upper left corner of the retainer.

What should I pay attention to when transferring my reporter gene set up from a cell line to a primary cell?

In general the overall expression level differs between different cell types. Don't expect the same level of gene induction when working with primary cells. You might need to change to other DNA amounts or ratios.

Do any of the three instruments used in the 96-well Shuttle System "go to sleep" or power off automatically?

The energy options of the laptop are set as such that only the screensaver is activated, the harddisk won't turn down and hibernation is disabled. One can change these settings via the system control. However this is not recommended because by...

What is the molecular weight of your control plasmid pmaxGFP?

The molecular weight is approximately 2.3x10e6 g/mol based on the average MW of a base pair being 660 g/mol.


Where can I find what the error codes on the Nucleofector or 96-well Shuttle mean?

In the back of the respective manuals, there is a chart giving a brief description of the error codes. If more information is needed or an error code is recurrent, it is best to contact Scientific Support for advice to determine if there is a...

According to which standard is the Nucleofector device certified?

The Nucleofector II was tested by TÜV Rheinland product safety GmbH and found to be in compliance with the following standards: IEC 61010-1:2001 (2nd edition) and EN 61010-1:2001 (2nd edition). TÜV Rheinland of North America Inc. tested the...
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