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726 results sorted by
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Healthy cells in culture contain maximal amounts of ATP and a wide range of metabolic stimuli do not increase these ATP levels. Any increase in the ATP concentration of a culture can therefore be associated with an increase in the number of...
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The lysis of the cells with NRR should not affect the DNA so whilst we haven't conducted this experiment ourselves I cannot see any real reason why it should not be possible to extract the DNA after lysis
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Yes. Since the ATP levels are precisely maintained in healthy cells, the level of ATP accurately reflects the number of viable cells in a culture. In response to a proliferative signal (e.g. a growth factor) the number of cells in a...
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There should be no problem with the use of the ViaLight kit with immobilized antibodies. You can if you wish confirm this with your own experiment by conducting a control experiment as follows:Coat triplicate wells of a 96 well plate with each...
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To achieve optimal performance and convenience a microplate luminometer should be used. If reagent injectors are available on the instrument, these should be used since they enable full automation of the assay. If reagent injectors are...
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Yes, activated T cells do have slightly higher ATP levels than resting, however, when you titrate out the cell number there are no significant differences. i.e. the increase in ATP per cell upon activation is nowhere near as great as the effect of...
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The sensitivity range of the reaction reported is in moles rather than molar and thus as you are assaying 100ml of your molar solution you will have ten times less ATP when measured in actual moles i.e. your lowest detectable level is actually...
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We have compared the ViaLight kits to all other assays routinely used in cell viability studies and have found the performance to be superior in every case. This is due to the combination of extreme sensitivity and wide dynamic range of...
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When conducting the ViaLight MDA assay I have used Luria Bertani broth for my cultures. It is best if the culture media is ATP free but if it is included as the negative control on the experimental plate then this will give you an idea of the...
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If you are worried that your product could contribute to the assay if you can add this to 100ul broth and conduct the assay as normal i.e. add 100ul of Bactolyse, wait 5 min and then add 20ul of AMR taking and immediate 1s integrated reading then...
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