Data Type
Cell Information
(849)
Citations
(4675)
FAQ
(685)
Culture Media
(92)
Category
Primary Cells and Media
(267)
Transfection
(178)
Laboratory Instrumentation
(69)
Electrophoreses and Analysis
(55)
Bioassay
(48)
3D Cell Culture
(42)
Live Cell Imaging
(34)
Mycoplasma Detection and Prevention
(29)
Endotoxin Detection
(27)
Serum-free and Speciality Media
(11)
Classical Media and Reagents
(10)
Cell Lines and Primary Cancer Cells
(7)
Uncategorized
(7)
Bioprocess Containers
(1)
Cell Services
(1)
+ Show All
Research Area
Uncategorized
(173)
Basic Research
(147)
Cancer Research/Cell Biology
(84)
Immunotherapy / Hematology
(68)
Stem Cells
(54)
Molecular Biology
(40)
Neurobiology
(38)
Respiratory Research
(31)
Toxicology
(29)
Endotoxin Testing
(27)
Cardiovascular
(26)
Regenerative medicine
(24)
Dermatology/Tissue Engineering
(21)
Gene Expression
(14)
Gastroenterology
(4)
Parasitology
(3)
+ Show All
685 results sorted by
relevance
alphabetical
newest first
oldest first
C
a
n
I
u
s
e
a
N
u
c
l
e
o
f
e
c
t
o
r
™
I
o
r
N
u
c
l
e
o
f
e
c
t
o
r
™
2
b
D
e
v
i
c
e
t
o
g
e
t
h
e
r
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
?
No, the 96-well Shuttle™ only works in conjunction with the Nucleofector™ IIs Device or our 4D-Nucleofector™ Device.
I
s
i
t
p
o
s
s
i
b
l
e
t
o
u
s
e
d
i
f
f
e
r
e
n
t
s
u
b
s
t
r
a
t
e
s
(
e
.
g
.
s
i
R
N
A
d
u
p
l
e
x
e
s
)
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
?
Yes. Transfection can be done with any substrate AND under exactly the same conditions. For example, the same conditions (96-well Shuttle™ Solution and program) are used for siRNA transfection or shRNA vectors. This greatly facilitates the...
I
s
i
t
p
o
s
s
i
b
l
e
t
o
p
r
e
-
p
l
a
t
e
s
u
b
s
t
r
a
t
e
s
w
h
e
n
u
s
i
n
g
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
Yes, pre-plating of substrates like siRNA and subsequent storage at -20°C is possible.
W
h
a
t
i
s
t
h
e
a
d
v
a
n
t
a
g
e
o
f
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
o
v
e
r
c
o
m
m
o
n
l
i
p
o
f
e
c
t
i
o
n
,
e
s
p
e
c
i
a
l
l
y
i
n
a
h
i
g
h
t
h
r
o
u
g
h
p
u
t
f
r
a
m
e
w
o
r
k
?
Several primary cells of high research relevance can simply not be transfected at relevant efficiencies and viabilities using lipofection. The same holds true for many suspension cell lines which are often used for protein production. These...
W
h
a
t
k
i
n
d
o
f
p
r
o
t
o
c
o
l
y
o
u
r
e
c
o
m
m
e
n
d
f
o
r
s
t
i
m
u
l
a
t
i
o
n
o
f
h
u
m
a
n
T
c
e
l
l
s
?
For getting the results we present in our datasheet, we recommend the follwing strategy: Cells should be stimulated and cultivated for 3 days before Nucleofection, preferentially on plates coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibody....
W
h
a
t
a
r
e
t
h
e
c
o
n
t
e
n
t
s
o
f
a
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
K
i
t
?
As the system will most likely be used for high-throughput approaches we provide plates which are pre-equipped with 96-well Nucleocuvette™ modules (2x8). In addition to that, the 96-well Shuttle solution, the supplement, and the pmaxGFP™ are also...
W
h
a
t
i
s
t
h
e
a
d
v
a
n
t
a
g
e
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
T
e
c
h
n
o
l
o
g
y
o
v
e
r
c
o
m
m
o
n
e
l
e
c
t
r
o
p
o
r
a
t
i
o
n
(
s
y
s
t
e
m
s
)
?
High cell viability and high transfection efficiency with the Nucleofection™ Technology are the most prominent features. Nucleofection™, i.e., the transfer of DNA into the nucleus and not only the cytosol, is essential when working with non-dividing...
A
r
e
t
h
e
r
e
a
n
y
e
f
f
e
c
t
s
o
n
t
h
e
d
i
f
f
e
r
e
n
t
i
a
t
i
o
n
o
f
n
e
u
r
a
l
s
t
e
m
c
e
l
l
s
f
o
l
l
o
w
i
n
g
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
The ability of transfected rat or mouse neural stem cells to be subsequently differentiated into a variety of cell types is well documented in: Olig2 Overexpression Induces the In Vitro Differentiation of Neural Stem Cells into Mature...
C
a
n
c
a
l
c
i
u
m
i
n
f
l
u
x
i
s
s
u
e
s
a
f
f
e
c
t
v
i
a
b
i
l
i
t
y
o
f
h
u
m
a
n
M
e
s
e
n
c
h
y
m
a
l
S
t
e
m
C
e
l
l
s
(
h
M
S
C
)
?
Yes. When cells are transfected using Nucleofection™, transient pores are generated in the cell membrane. Generally, these pores disappear within 15 minutes of transfection, but if cells are plated immediately after Nucleofection™ in media that...
H
o
w
m
u
c
h
D
N
A
s
h
o
u
l
d
I
u
s
e
i
n
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
™
R
e
a
c
t
i
o
n
t
o
o
b
t
a
i
n
s
t
a
b
l
e
c
l
o
n
e
s
?
You should use the amount of DNA recommended in the optimized protocol for transient transfection. If obtaining a very high number of clones is important for your experiments, you can try increasing the amount of DNA. For further tips see our...
PREVIOUS
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
PAGE
12
NEXT