Data Type
Cell Information
(855)
Citations
(4783)
FAQ
(703)
Culture Media
(92)
Category
Primary Cells and Media
(276)
Transfection
(185)
Laboratory Instrumentation
(72)
Electrophoreses and Analysis
(56)
Endotoxin Detection
(56)
Bioassay
(48)
3D Cell Culture
(42)
Mycoplasma Detection and Prevention
(34)
Serum-free and Speciality Media
(15)
Classical Media and Reagents
(11)
Cell Lines and Primary Cancer Cells
(9)
Live Cell Imaging
(8)
Uncategorized
(7)
Cell Services
(3)
Bioprocess Containers
(1)
Protozoa
(1)
+ Show All
Research Area
Uncategorized
(174)
Basic Research
(130)
Cancer Research/Cell Biology
(85)
Immunotherapy / Hematology
(71)
Endotoxin Testing
(55)
Stem Cells
(53)
Molecular Biology
(40)
Neurobiology
(38)
Respiratory Research
(33)
Toxicology
(33)
Cardiovascular
(28)
Regenerative medicine
(24)
Dermatology/Tissue Engineering
(21)
Gene Expression
(15)
Drug Discovery
(7)
Gastroenterology
(4)
Parasitology
(3)
+ Show All
703 results sorted by
relevance
alphabetical
newest first
oldest first
W
h
e
r
e
c
a
n
I
f
i
n
d
t
h
e
e
x
p
i
r
a
t
i
o
n
d
a
t
e
s
f
o
r
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
K
i
t
a
n
d
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
S
o
l
u
t
i
o
n
?
The expiration dates are located on the outside label of the kit box and on the label of the solution box.
W
h
a
t
i
s
t
h
e
p
u
r
p
o
s
e
o
f
t
h
e
5
-
b
r
o
m
o
-
2
'
-
d
e
o
x
y
u
r
i
d
i
n
e
(
B
r
d
U
)
w
h
e
n
c
u
l
t
u
r
i
n
g
r
a
t
c
a
r
d
i
a
c
m
y
o
c
y
t
e
s
?
BrDU is very efficient at inhibiting the proliferation of fibroblasts which are natively present at the time of isolation. Without the addition of the BrDU, the fibroblasts would overtake the culture.
H
o
w
m
a
n
y
p
a
s
s
a
g
e
s
c
a
n
I
u
s
e
t
h
e
C
l
o
n
e
t
i
c
s
™
a
n
d
D
i
s
e
a
s
e
d
C
e
l
l
s
?
Proliferative capacity is tested through one or more passages (depending on the cell type) for information only and is NOT a specification where a minimum number of doublings must be obtained in order to pass our testing unlike the normal counterpart...
C
a
n
a
n
a
l
t
e
r
n
a
t
e
t
o
t
h
e
5
-
b
r
o
m
o
-
2
'
-
d
e
o
x
y
u
r
i
d
i
n
e
(
B
r
d
U
)
b
e
u
s
e
d
t
o
c
o
n
t
r
o
l
t
h
e
g
r
o
w
t
h
o
f
f
i
b
r
o
b
l
a
s
t
s
w
h
e
n
c
u
l
t
u
r
i
n
g
r
a
t
c
a
r
d
i
a
c
m
y
o
c
y
t
e
s
?
At this time, we can only recommend the use of BrDU for controlling the growth of fibtoblasts.When developing this product, we tried using cytosine ?-arabino furanoside (CAF) to inhibit fibroblast growth. While CAF successfully inhibited the growth...
D
o
w
e
k
n
o
w
t
h
e
s
e
v
e
r
i
t
y
o
f
t
h
e
a
s
t
h
m
a
o
r
t
h
e
g
e
n
o
t
y
p
e
o
f
t
h
e
c
y
s
t
i
c
f
i
b
r
o
s
i
s
d
o
n
o
r
s
?
"Generally, information on the severity of asthma is not available. For our diseased cells from donors with cystic fibrosis, we usually have information on the genotype of the donor. Please contact our scientific support department for further...
C
a
n
a
n
a
l
t
e
r
n
a
t
e
t
o
t
h
e
n
i
t
r
o
c
e
l
l
u
l
o
s
e
b
e
u
s
e
d
a
s
a
n
e
x
t
r
a
c
e
l
l
u
l
a
r
m
a
t
r
i
x
w
h
e
n
c
u
l
t
u
r
i
n
g
r
a
t
c
a
r
d
i
a
c
m
y
o
c
y
t
e
s
?
Lonza’s rat cardiac myocytes can also be cultured on fibronectin or laminin. Nitrocellulose was selected as the recommended substrate for its convenience and ease of use.
D
o
I
n
e
e
d
a
s
p
e
c
i
a
l
m
e
d
i
a
o
r
p
r
o
t
o
c
o
l
f
o
r
c
u
l
t
u
r
i
n
g
t
h
e
C
l
o
n
e
t
i
c
s
™
a
n
d
P
o
i
e
t
i
c
s
™
D
i
s
e
a
s
e
d
C
e
l
l
s
?
No, all of our diseased cells are isolated, expanded, and tested in the same media and using the same protocol as the normal counterpart cells.
H
o
w
l
o
n
g
c
a
n
t
h
e
r
a
t
c
a
r
d
i
a
c
m
y
o
c
y
t
e
s
s
u
r
v
i
v
e
i
n
c
u
l
t
u
r
e
?
Lonza has observed these cells up to 59 days in culture and even at that time, the cells displayed beating in syncytium. It is, however, possible that unseen physiological changes may occur to the cells during this time. As these cells are a...
T
h
e
S
a
r
s
t
e
d
t
t
u
b
e
s
(
C
a
t
.
N
o
.
5
5
.
4
7
8
)
t
h
a
t
y
o
u
r
e
c
o
m
m
e
n
d
f
o
r
u
s
e
w
i
t
h
L
u
c
e
t
t
a
h
a
v
e
b
e
e
n
d
i
s
c
o
n
t
i
n
u
e
d
.
W
h
a
t
a
l
t
e
r
n
a
t
i
v
e
s
t
u
b
e
s
c
a
n
I
u
s
e
?
Alternatively, you may use Sarstedt tubes (Cat. No. 55.476) or BD Falcon Round-Bottom Tubes (Cat. No. 352054). You may also use other tubes which fulfill following specifications: Polystyrene tubes of 75 mm length and up to 12 mm diameter. Tubes...
C
a
n
I
t
e
s
t
c
e
l
l
c
u
l
t
u
r
e
s
u
p
e
r
n
a
t
a
n
t
w
i
t
h
T
o
x
i
L
i
g
h
t
™
a
n
d
V
i
a
L
i
g
h
t
™
?
Yes. Supernatant for ToxiLight™ Assay can be transferred to a luminescence compatible format prior to beginning assay. Supernatant for ViaLight™ Assay can be transferred to a luminescence compatible format only after the cell lysis step has...
PREVIOUS
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
PAGE
12
NEXT