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673 results sorted by
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According to QBM Cell Sciences, you can expect 12 million cortical neurons from an average E18-E19 rat pup. You can expect 4 million cortical neurons from an average mouse pup.
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No, it does not recognize the orientation of the plate. Please make sure that well A1 is positioned in the upper left corner of the retainer.
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The energy options of the laptop are set as such that only the screensaver is activated, the harddisk won't turn down and hibernation is disabled. One can change these settings via the system control. However this is not recommended because by...
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The molecular weight is approximately 2.3x10e6 g/mol based on the average MW of a base pair being 660 g/mol.
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The molecular weight of the protein is about 27kDa.
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The original attenuation of the IRES sequence was performed to allow for a greater difference between the expression levels of the upstream gene of interest and the downstream reporter gene. If the downstream reporter gene, the product of less than...
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As the stability and half-life of various mRNAs and their protein products varies, it is important to empirically determine the best time points for assessing target knockdown. For example, it has been documented that in mammalian cells, mRNA...
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Yes. As the same protocol applies for any nucleic acid substrate (vectors or oligonucleotides) you can easily perform co-transfections either for transfection control or rescue experiments.
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For optimal stimulation of the transfected cells we recommend coating 96-wells plates for stimulation [Nunc Immuno™ Plate C96 Maxi Sorp™, Cat. No. 430 341] with 50 µl of a solution of anti- CD3 antibody [OKt 3; eBioscience, Cat. No. 14-0037-82;...
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The reason is that insect cells are often incubated in very simple incubators that are not humidified (and without CO 2 at 24°C to 28°C). Insect media typically do not need CO 2 for buffering pH, so gas exchange is not required. To avoid...
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