Search Results

How much DNA should I use in my Nucleofection® Reaction to obtain stable clones?

You should use the amount of DNA recommended in the optimized protocol for transient transfection. If obtaining a very high number of clones is important for your experiments, you can try increasing the amount of DNA. For further tips see our...

Why is the Nucleofector® Technology ideal for protein production from stable clones ?

The generation of a stable clone often requires six months or more due to selection procedures and adaption to serum-free conditions after transfection.With the help of the Nucleofector® Technology suspension cells can be transfected directly in...

For Lonza's animal neurons, how many cortical neurons can I expect from a single pup?

According to our manufacturer, you can expect 12 million cortical neurons from an average E18-E19 rat pup.You can expect 4 million cortical neurons from an average mouse pup.

Can I use the pmaxGFP™ control plasmid with insect cells such as S2 cells or SF9 cells?

The pmaxGFP™ Vector provided in our Nucleofector® Kits is not expressed in insect cells. We strongly recommend an insect expression vector encoding a fluorescent protein or lacZ reporter as a positive control for your experiments [e.g. Novagen™’s...

Do you have any information regarding differentiation of mouse ES cells after Nucleofection® with large plasmids?

It is unlikely that plasmid size would affect in vitro differentiation capability. Gene targeting constructs are often as large as 20 kb and if treated well the ES cells are perfectly capable of generating germ line chimaeras afterwards (indicating...

Does the GFP sequence in the pmaxGFP™ control plasmid has a stop codon?

Yes. The coding sequence ends with a stop codon (TGA/UGA).

Does the Mouse T cell protocol work for stimulated mouse T cells?

Yes. Stimulate the cells with anti CD3 and anti CD28 antibody for 24 hours. Use the optimized protocol. Often times, this will result in higher transfection efficiency (60-70% is possible). Alternatively, you can stimulate with ConA and IL-2 for 2-3...

What antibodies can be used against the GFP produced by pmaxGFP™ and pmaxFP™-Green vectors? What antibodies can be used to detect denatured GFP? What antibodies can be used to detect the RFP expressed by pmaxFP-Red?

Most of the commercial antibodies for GFP do not detect GFP expressed from pmaxGFP or pmaxFP-Green vectors. The origin of the protein expressed by the pmaxGFP control plasmid and pmaxFP-Green is from the green fluorescent protein CopGFP, cloned from...

What is the molecular weight (MW) of your control plasmid pmaxGFP™?

The molecular weight is approximately 2.3x10e6 g/mol based on the average MW of a base pair being 660 g/mol.

What is the molecular weight (MW) of the GFP protein expressed by your control pmaxGFP™ plasmid?

The molecular weight of the protein is about 27kDa.

Data Type

Category

Research Area