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515 results sorted by
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H
o
w
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p
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®
o
f
n
e
u
r
o
n
s
?
The quality and the concentration of DNA used for Nucleofection® in general plays a central role for the efficiency of gene transfer. We strongly recommend using endotoxin free prepared DNA. Endotoxin free Kits are available from several...
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a
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®
I
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9
6
-
w
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S
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t
t
l
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®
D
e
v
i
c
e
?
No, the 96-well Shuttle® only works in conjunction with the Nucleofector® IIs Device or our 4D-Nucleofector® Device.
W
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t
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N
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®
T
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p
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n
(
s
y
s
t
e
m
s
)
?
High cell viability and high transfection efficiency with the Nucleofection® Technology are the most prominent features. Nucleofection®, i.e., the transfer of DNA into the nucleus and not only the cytosol, is essential when working with...
I
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N
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®
P
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m
u
s
i
n
g
.
W
h
y
?
The duration time of a program is dependent on the number of the program. The duration is also dependent on how long the Nucleofector® System has been turned on as well as the number of Nucleofections you have done in the last couple of...
W
h
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t
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m
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(
M
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p
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m
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p
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x
G
F
P
™
?
The molecular weight is approximately 2.3x10e6 g/mol based on the average MW of a base pair being 660 g/mol.
W
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m
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G
F
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™
p
l
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s
m
i
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?
The molecular weight of the protein is about 27kDa.
W
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D
N
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w
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n
?
D
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I
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d
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D
N
A
c
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e
r
f
o
r
l
o
w
D
N
A
a
m
o
u
n
t
s
?
The recommended amount of DNA per 100 µl reaction is 0.5µg-5 µg. No carrier is needed.
I
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p
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9
6
-
w
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S
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®
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
Yes, pre-plating of substrates like siRNA and subsequent storage at -20°C is possible.
C
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l
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f
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N
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™
R
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a
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?
C
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c
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t
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m
o
r
e
t
h
a
n
o
n
c
e
?
No. The electrical parameters provided by the Nucleofector™ Device are optimized for the cuvettes contained in the Nucleofector™ Kits. The cuvettes are single-use only. Using the cuvettes more than once will result in higher cell death and lower...
I
f
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e
M
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b
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d
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f
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N
u
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o
f
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c
t
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o
n
®
r
e
s
u
l
t
s
?
No, it seems that the beads bound to the cell surface do not have any influence on Nucleofection®. Larger magnetic beads (i.e. > 1µm) may disturb the Nucleofection® process and negatively influence cell viability and efficiency.
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