Data Type
Cell Information
(850)
Citations
(4689)
FAQ
(684)
Culture Media
(92)
Category
Primary Cells and Media
(268)
Transfection
(178)
Laboratory Instrumentation
(69)
Electrophoreses and Analysis
(55)
Bioassay
(47)
3D Cell Culture
(42)
Live Cell Imaging
(34)
Mycoplasma Detection and Prevention
(30)
Endotoxin Detection
(27)
Serum-free and Speciality Media
(11)
Classical Media and Reagents
(10)
Cell Lines and Primary Cancer Cells
(8)
Uncategorized
(7)
Bioprocess Containers
(1)
Cell Services
(1)
+ Show All
Research Area
Uncategorized
(173)
Basic Research
(146)
Cancer Research/Cell Biology
(84)
Immunotherapy / Hematology
(68)
Stem Cells
(54)
Molecular Biology
(40)
Neurobiology
(38)
Respiratory Research
(31)
Toxicology
(29)
Endotoxin Testing
(27)
Cardiovascular
(26)
Regenerative medicine
(24)
Dermatology/Tissue Engineering
(21)
Gene Expression
(14)
Gastroenterology
(4)
Parasitology
(3)
+ Show All
684 results sorted by
relevance
alphabetical
newest first
oldest first
C
a
n
I
u
s
e
l
a
r
g
e
r
c
u
v
e
t
t
e
s
f
o
r
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
™
R
e
a
c
t
i
o
n
?
C
a
n
I
u
s
e
t
h
e
c
u
v
e
t
t
e
s
m
o
r
e
t
h
a
n
o
n
c
e
?
No. The electrical parameters provided by the Nucleofector™ Device are optimized for the cuvettes contained in the Nucleofector™ Kits. The cuvettes are single-use only. Using the cuvettes more than once will result in higher cell death and lower...
W
h
i
c
h
p
i
p
e
t
t
e
t
i
p
s
a
r
e
c
o
m
p
a
t
i
b
l
e
w
i
t
h
t
h
e
&
n
b
s
p
;
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
N
u
c
l
e
o
c
u
v
e
t
t
e
™
P
l
a
t
e
s
?
We recommend using special tips, e.g. epT.I.P.S™ (Eppendorf, Hamburg, Germany) or TallTips™ (Matrix Technologies, Hudson, NH, USA). You can contact our Scientific Support Team for a list of compatible tips, including tips for pipetting robots.
I
f
I
u
s
e
M
i
l
t
e
n
y
i
p
a
r
a
m
a
g
n
e
t
i
c
b
e
a
d
s
f
o
r
p
o
s
i
t
i
v
e
s
e
l
e
c
t
i
o
n
o
f
c
e
l
l
s
,
i
.
e
.
b
e
a
d
s
r
e
m
a
i
n
o
n
t
h
e
c
e
l
l
s
u
r
f
a
c
e
,
d
o
e
s
t
h
a
t
i
n
f
l
u
e
n
c
e
N
u
c
l
e
o
f
e
c
t
i
o
n
™
r
e
s
u
l
t
s
?
No, it seems that the beads bound to the cell surface do not have any influence on Nucleofection™. Larger magnetic beads (i.e. > 1µm) may disturb the Nucleofection™ process and negatively influence cell viability and efficiency.
C
a
n
I
f
r
e
e
z
e
t
h
e
9
6
-
w
e
l
l
N
u
c
l
e
o
c
u
v
e
t
t
e
™
P
l
a
t
e
s
?
Yes. Freezing of the plates at -20°C does not alter their properties.
W
h
a
t
i
s
t
h
e
p
r
o
o
f
t
h
a
t
D
N
A
e
n
t
e
r
s
t
h
e
n
u
c
l
e
u
s
d
u
r
i
n
g
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
There are two facts that prove that the DNA is directly transported into the nucleus: 1.) Using the Nucleofector™ Technology, transfection of non-dividing cells is possible. With conventional non-viral transfection methods cell division is a...
C
a
n
I
i
n
t
e
g
r
a
t
e
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
i
n
t
o
a
l
i
q
u
i
d
h
a
n
d
l
i
n
g
s
y
s
t
e
m
?
Yes. The 96-well Shuttle™ Device is designed in such a way that it is addressable by robot grippers. Furthermore, the software contains the required interface to be connected with the LHS software. However, the practical implementation for each...
H
o
w
c
a
n
I
r
e
m
o
v
e
t
h
e
c
e
l
l
s
f
r
o
m
t
h
e
c
u
v
e
t
t
e
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
o
r
™
?
I
s
t
h
e
r
e
a
n
y
a
l
t
e
r
n
a
t
i
v
e
?
We recommend using the plastic pipettes provided in the kits. These pipettes prevent any damage to the cells and ensure a good cell recovery.
C
a
n
I
u
s
e
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
2
b
S
o
l
u
t
i
o
n
s
a
n
d
O
p
t
i
m
i
z
e
d
P
r
o
t
o
c
o
l
s
w
i
t
h
t
h
e
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
™
X
-
U
n
i
t
o
r
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
?
No. The 4D-Nucleofector and 96-well Shuttle™ System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle™ Solutions are available for primary cells. For cell lines, three different...
C
a
n
I
u
s
e
a
N
u
c
l
e
o
f
e
c
t
o
r
™
I
o
r
N
u
c
l
e
o
f
e
c
t
o
r
™
2
b
D
e
v
i
c
e
t
o
g
e
t
h
e
r
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
?
No, the 96-well Shuttle™ only works in conjunction with the Nucleofector™ IIs Device or our 4D-Nucleofector™ Device.
W
h
a
t
m
e
t
h
o
d
c
a
n
I
u
s
e
t
o
f
i
x
m
y
H
L
-
6
0
c
e
l
l
s
a
s
s
h
o
w
n
i
n
y
o
u
r
o
p
t
i
m
i
z
e
d
p
r
o
t
o
c
o
l
?
The cells which are shown in the HL-60 optimized protocol are living cells and not fixed cells. However a good method for fixation of GFP is to use 3.5%-4% Paraformaldehyde in PBS. Please keep in mind that the fixation method is dependant on what...
PREVIOUS
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
NEXT