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This depends on the size of the incubator and whether the pump is kept inside or outside the incubator. We have fit 18 2-or-more chamber systems into one incubator containing two pumps.
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Fresh supernatant and testing the same day is still the recommended procedure as mycoalert wasdesigned as a fast preliminary screen for mycoplasma. For this the supernatant can be kept at roomtemperature or 4oC until the end of the day before...
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Cells are seeded onto cell culture inserts or scaffolds in a static 24-well plate and cultured until confluent. System components (chambers, reservoir bottle, and tubing) are autoclaved, and systems are connected the day before and washed with PBS...
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Gentomycin and all commonly used antiboitics do not effect MycoAlert. We have tested amphotericin, ampicillin, cephalothin,kanamycin, lincomycin, in addition to gentomycin, penicillin and streptomycin and none interfere with theMycoAlert Assay.
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A low pH can certainly affect the luciferase system used In all of these products. The luciferase will not work if the pH is below 6.5 and performance will rapidly decay below pH 7.2. The reagents in these kits will buffer back to some degree but...
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Hepatocytes have been cultured for a month and have retained their phenotype and CYP gene expression. The length of the experiment can be varied from 1 to 3 day experiments, to week or month-long studies and potentially longer.
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Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells.The MycoZap™ Reagent eliminates mycoplasma...
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The Quasi Vivo™ system is highly flexible and can be configured for a wide range of experimental designs. Different chamber models can also be connected, to model interactions between cell types in different environments.
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The Nucleofector™ Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell-type...
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There are several reasons to choose the Nucleofector™ Technology for your gene transfer experiments. One is the direct transfer of DNA to the cell nucleus. This makes gene expression independent from cell division. Therefore, the technique is the...
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