Q:

Four hours after Nucleofection™, I can see the hepatocytes have attached to the well, but the morphology does not look correct, should I be concerned?

A:

There is no reason for alarm. The hepatocytes may not exhibit normal morphology a few hours after Nucleofection™ but by 24 hours post Nucleofection™, morphology should be normal. Remember to perform a fluid change about 4 hours post Nucleofection™.

Categories:
Primary Cells and Media
Transfection
Research Areas:
Toxicology