faq

Q:	How can I detect mycoplasma contamination of my cells?
A:	We recommend using the 20-minute MycoAlert® Mycoplasma Detection Kit from Lonza. The simple four-step MycoAlert® Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid screening procedure allowing sensitive detection of contaminating mycoplasma in a test sample. The viable mycoplasma are lysed and the enzymes react with the MycoAlert® Substrate catalyzing the conversion of ADP to ATP. By measuring the level of ATP in a sample both before and after the addition of the MycoAlert® Substrate a ratio can be obtained which is indicative of the presence or absence of mycoplasma.

How can I detect mycoplasma contamination of my cells?

We recommend using the 20-minute MycoAlert® Mycoplasma Detection Kit from Lonza.

The simple four-step MycoAlert® Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid screening procedure allowing sensitive detection of contaminating mycoplasma in a test sample. The viable mycoplasma are lysed and the enzymes react with the MycoAlert® Substrate catalyzing the conversion of ADP to ATP. By measuring the level of ATP in a sample both before and after the addition of the MycoAlert® Substrate a ratio can be obtained which is indicative of the presence or absence of mycoplasma.

Categories:

Mycoplasma Detection and Prevention

Research Areas:

Basic Research