Q:

What antibodies can be used against the GFP produced by pmaxGFP™ and pmaxFP™-Green vectors? What antibodies can be used to detect denatured GFP? What antibodies can be used to detect the RFP expressed by pmaxFP-Red?

A:
Most of the commercial antibodies for GFP do not detect GFP expressed from pmaxGFP or pmaxFP-Green vectors. The origin of the protein expressed by the pmaxGFP control plasmid and pmaxFP-Green is from the green fluorescent protein CopGFP, cloned from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda). Most commercial antibodies for GFP are designed to react with proteins isolated from other organisms. For example, Clontech`s GFP is from Aequorea victoria (jellyfish), and would not react with antibodies to pmaxGFP or pmaxFP-Green. Evrogen offers antibodies against the copepod GFP which can be used to detect the GFP expressed by our vectors: >Anti-GFP, Cat. No. AB501 and AB502 are used against non-denatured protein produced by pmaxGFP >Anti-TurboGFP antibodies Cat. No. AB511 and AB512 are used against non-denatured protein expressed from pmaxFP-Green >Anti-TurboGFP(d) antibodies Cat. No. AB513 and AB514 will recognize denatured protein expressed from both pmaxGFP and pmaxFP-Green vectors. The pmaxFP-Red is identical to JRed from Evrogen. For detection of the RFP expressed by pmaxFP-Red, the Anti-KillerRed antibody from Evrogen, catalog numbers AB961 and AB962, will need to be used. For more information about these antibodies, please visit Evrogen's website under related links. In the US, Evrogen products are available through Axxora, link also in related links.
Categories:
Transfection
Research Areas:
Uncategorized