We recommend allowing the transfected hepatocytes to recover in Plating medium for 72 hours post-Nucleofection®.

For induction studies (e.g. CYP3A4), the Plating medium is replaced with maintenance medium (William's E without FCS and EGF) and Rifampicin is added for an additional 48 hours.

The expression of CYP3A4 is analyzed 120 hours post-Nucleofection.