Q:

In stable cell line generation, I have a very high transfection efficiency, but most of my cells die during selection. Is this to be expected?

A:

This is the normal pattern you should expect to see. Only a small proportion (1/10,000 to 1/100) of all transfected cells will integrate the transfected DNA into their genome and become stable transfectants. The remaining cells lose the transfected DNA and stop expressing relatively quickly.

Categories:
Transfection
Research Areas:
Gene Expression