Q:

I have a very high transfection efficiency but most of my cells die during selection, is this to be expected?

A:
This is the normal pattern you should expect to see. Only a small proportion (1/10,000 to 1/100) of all transfected cells will integrate the transfected DNA into their genome and become stable transfectants. The remaining cells lose the transfected DNA and stop expressing relatively quickly.
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Categories:
Transfection
Research Areas:
Uncategorized