Q:

Do you have any information regarding differentiation of mouse ES cells after Nucleofection™ with large plasmids?

A:

It is unlikely that plasmid size would affect in vitro differentiation capability. Gene targeting constructs are often as large as 20 kb and if treated well the ES cells are perfectly capable of generating germ line chimaeras afterwards (indicating they have retained their full differentiation potential).

Categories:
Transfection
Research Areas:
Immunotherapy / Hematology
Stem Cells
Basic Research