Embryonic stem cell (ES), mouse

Stem cells derived from mouse blastocyst.

Cell Type:
Embryonic SC
Tissue Origin:
embryo
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Stem Cells
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 96-CG-104 5e4 85-95% 58-88% Plasmid (general) 0.4 µg 20 µl Shuttle
ES cell, mouse A-013 5e6 80% Plasmid (general) 9 µg 100 µl I/II/2b
P3

CG-104

5e4 85-95% 58-88% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (30)

Categories:
Primary Cells and Media, Transfection 
Authors:
Dewari PS, Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki M, Blin C, Finch R, Bressan RB, Morrison G, Jacobi AM, Behlke MA, von Kriegsheim A, Tomlinson S, Krijgsveld J, Pollard SM. 
In:
eLife (2018) 11;7: 1-29 
Categories:
Transfection 
Authors:
Minyan Li, Rui Huang, Xue Jiang, Yuxi Chen, Zhen Zhang, Xiya Zhang, Puping Liang, Shaoquan Zhan, Shanbo Cao, Zhou Songyang mail, Junjiu Huang 
In:
PLoS ONE (2015) 10(11): e0143148 
Categories:
Transfection 
Authors:
Li K1, Wang G1, Andersen T2, Zhou P1, Pu WT3. 
In:
PLoS ONE (2014) 9(8): 1-10 
Categories:
 
Authors:
Sun H, Quan Y, Yan Q, Peng X, Mao Z, Wetsel RA, Wang D. 
In:
Tissue Eng (2014) 20(6): 464-72 
Categories:
Transfection 
Authors:
Carbery ID, Ji D, Harrington A, Brown V, Weinstein EJ, Liaw L, Cui X 
In:
Genome Res (2010) 186(2): 451-9 
Categories:
Transfection 
Authors:
D'Aniello C, Lonardo E, Iaconis S, Guardiola O, Liguoro AM, Liguori GL, Autiero M, Carmeliet P, Minchiotti G 
In:
Circ Res (2009) 105(3): 211-3 
Categories:
Transfection 
Authors:
Margariti A, Xiao Q, Zampetaki A, Zhang Z, Li H, Martin D, Hu Y, Zeng L, Xu Q 
In:
J Cell Sci (2009) 122(Pt 4): 460-70 
Categories:
Transfection 
Authors:
Vincenz C, Kerppola TK 
In:
Proc Natl Acad Sci USA (2008) 105(43): 16572-7 
Categories:
Transfection 
Authors:
Tarasov KV, Tarasova YS, Tam WL, Riordon DR, Elliott ST, Kania G, Li J, Yamanaka S, Crider DG, Testa G, Li RA, Lim B, Stewart CL, Liu Y, Van Eyk JE, Wersto RP, Wobus AM, Boheler KR 
In:
PLoS ONE (2008) 3(6): e2478 
Categories:
Transfection 
Authors:
Singh SK, Kagalwala MN, Parker-Thornburg J, Adams H, Majumder S 
In:
Nature (2008) 453(7192): 223-7 
Categories:
Transfection 
Authors:
Kondo T, Sheets PL, Zopf DA, Aloor HL, Cummins TR, Chan RJ, Hashino E 
In:
Proc Natl Acad Sci USA (2008) 105(15): 5780-5 
Categories:
Transfection 
Authors:
Sinkkonen L, Hugenschmidt T, Berninger P, Gaidatzis D, Mohn F, Artus-Revel CG, Zavolan M, Svoboda P, Filipowicz W 
In:
Nat Struct Mol Biol (2008) 15(3): 259-67 
Categories:
Transfection 
Authors:
Rojek JM, Campbell KP, Oldstone MB, Kunz S 
In:
Mol Biol Cell (2007) 18(11): 4493-507 
Categories:
Transfection 
Authors:
Spencer HL, Eastham AM, Merry CL, Southgate TD, Perez-Campo F, Soncin F, Ritson S, Kemler R, Stern PL, Ward CM 
In:
Mol Biol Cell (2007) 18(8): 2838-51 
Categories:
Transfection 
Authors:
Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD 
In:
Nature (2007) 448(7150): 196-9 
Categories:
Transfection 
Authors:
Jeong CH, Lee HJ, Cha JH, Kim JH, Kim KR, Kim JH, Yoon DK, Kim KW 
In:
J Biol Chem (2007) 282(18): 13672-9 
Categories:
Transfection 
Authors:
Soto-Gutiérrez A, Navarro-Alvarez N, Zhao D, Rivas-Carrillo JD, Lebkowski J, Tanaka N, Fox IJ, Kobayashi N 
In:
Nat Protocols (2007) 2(2): 347-356 
Categories:
Transfection 
Authors:
Verani R, Cappuccio I, Spinsanti P, Gradini R, Caruso A, Magnotti MC, Motolese M, Nicoletti F, Melchiorri D 
In:
J Neurochem (2007) 100(1): 242-50 
Categories:
Transfection 
Authors:
Fukazawa T, Maeda Y, Durbin ML, Nakai T, Matsuoka J, Tanaka H, Naomoto Y, Tanaka N 
In:
Mol Cancer Ther (2007) 6(1): 244-52 
Categories:
Transfection 
Authors:
Donohoe ME, Zhang LF, Xu N, Shi Y, Lee JT 
In:
Mol Cell (2007) 25(1): 43-56 
Categories:
Transfection 
Authors:
Leitner NR, Strobl B, Bokor M, Painz R, Kolbe T, Rulicke T, Muller M, Karaghiosoff M 
In:
BMC Biotechnol (2006) 6: 48 
Categories:
Transfection 
Authors:
Soto-Gutierrez A, Kobayashi N, Rivas-Carrillo JD, Navarro-Alvarez N, Zhao D, Okitsu T, Noguchi H, Basma H, Tabata Y, Chen Y, Tanaka K, Narushima M, Miki A, Ueda T, Jun HS, Yoon JW, Lebkowski J, Tanaka N, Fox IJ 
In:
Nat Biotechnol (2006) 24(11): 1412-1419 
Categories:
Transfection 
Authors:
Chen S, Do JT, Zhang Q, Yao S, Yan F, Peters EC, Schöler HR, Schultz PG, Ding S 
In:
Proc Natl Acad Sci USA (2006) 103(46): 17266-71 
Categories:
Transfection 
Authors:
Spinsanti P, De Vita T, Di Castro S, Storto M, Formisano P, Nicoletti F, Melchiorri D 
In:
J Neurochem (2006) 99(1): 299-307 
Categories:
Transfection 
Authors:
Andersson E, Tryggvason U, Deng Q, Friling S, Alekseenko Z, Robert B, Perlmann T, Ericson J 
In:
Cell (2006) 124(2): 393-405 
Categories:
Transfection 
Authors:
Kobayashi N, Rivas-Carrillo JD, Soto-Gutierrez A, Fukazawa T, Chen Y, Navarro-Alvarez N, Tanaka N 
In:
Birth Defects Res C Embryo Today (2005) 75(1): 10-8 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rippon HJ, Ali NN, Polak JM, Bishop AE. 
In:
Cloning and Stem Cells (2004) 6(2): 49-56 
Categories:
Transfection 
Authors:
Conti MA, Even-Ram S, Liu C, Yamada KM and Adelstein RS 
In:
J Biol Chem (2004) 279(40): 41263-41266 
Categories:
Transfection 
Authors:
Lorenz P, Harnack U and Morgenstern R 
In:
Biotechnol Lett (2004) 26(20): 1589-1592 
Categories:
Transfection 
Authors:
Lakshmipathy U, Pelacho B, Sudo K, Linehan JL, Coucouvanis E, Kaufman DS and Verfaillie CM 
In:
Stem Cells (2004) 22(4): 531-543 
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