faq

Q:	What is the amount of DNA I can use in my Nucleofection® Experiment?
A:	This totally depends on the Nucleofector® system you are working with. While in the 100 µL reaction vessels usually 1 - 5 µg DNA is being used, for the 20 µL reaction vessels (e.g. X Unit, 96-well Unit and HT Nucleofector® System) 0.2 – 1 µg DNA is a good starting amount. Working with the 4D-Nucleofector® LV Unit is scaleable and therefore per 1 mL reaction about 40 µg should be calculated. Please ensure that the volume of substarte added should not exceed 10% of the total reaction volume to avoid significant dilution of the Nucleofector® Solution by substrate buffer. Alternatively, the substrate might be diluted by Nucleofector® Solution. Furthermore, some cells might be sensitive to larger amounts of DNA. Please check the cell specific Optimized Protocol (OP) for the recommended total DNA amount of the cell type of interest.

What is the amount of DNA I can use in my Nucleofection® Experiment?

This totally depends on the Nucleofector® system you are working with.

While in the 100 µL reaction vessels usually 1 - 5 µg DNA is being used, for the 20 µL reaction vessels (e.g. X Unit, 96-well Unit and HT Nucleofector® System)

0.2 – 1 µg DNA is a good starting amount.

Working with the 4D-Nucleofector® LV Unit is scaleable and therefore per 1 mL reaction about 40 µg should be calculated.

Please ensure that the volume of substarte added should not exceed 10% of the total reaction volume to avoid significant dilution of the Nucleofector® Solution by substrate buffer. Alternatively, the substrate might be diluted by Nucleofector® Solution.

Furthermore, some cells might be sensitive to larger amounts of DNA. Please check the cell specific Optimized Protocol (OP) for the recommended total DNA amount of the cell type of interest.

Categories:

Transfection

Research Areas:

Gene Expression