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Using the RAFT™ System, it takes less than 1 hour to create 3D collagen cell cultures – individually or in batches depending on your research requirements.
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The in-vitro fm can be calculated: - early in the development of the test compound, by using the Silensomes™ HLM of 3 key CYPs involved in the biotransformation of many drugs, CYP3A4 and CYP1A2, and CYP2D6. (Catalog numbers SIL200K, SIL210K, and...
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Growing cells in the RAFT™ 3D Culture System versus on any other coating including collagen, Matrigel™ or similar, comes to compare 3D to 2D cell culture. You might notice big differences in cells behavior and response. Cell-to-cell or -matrix...
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The RAFT™ System uses type I collagen, the major component of natural extracellular matrix (ECM), as it is the ideal material for forming realistic 3D cell cultures. The patented RAFT™ Process enables a physiologically relevant concentration of...
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Because the control Silensomes™ HLM must be made in the same production run as the inhibited Silensomes™ HLM, it’s important to compare the batch numbers on each vial. To determine whether the batch runs match, compare the last 4 digits of the lot...
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With the compression, the RAFT™ 3D Cell Culture System helsp to obtain high density collagens within a final concentration of 80 mg/ml that resemble to the in vivo tissue collagen density in the same magnitude. Classical hydrogels without compression...
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The RAFT™ Process allows the user to control the process of making 3D cell cultures. The RAFT™ System enables the researcher to determine the cell seeding density in their cultures and supports more complex cell co-culture. Cells can be cultured...
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Yes, we would initiate a custom Silensomes project with you to evaluate your current stock and create a project to develop the Silensomes™ from it. There is a certain amount of activity loss when performing the inhibition reactions, so you not be...
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Several papers have been published describing the results of using this novel process and a range of methods and analytical approaches have been used which show that the cell cultures produced mimic the cells natural environment.
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RAFT™ 3D Cell Cultures made according to the standard protocol have a thickness of approximately 100-120µm – slightly influenced by the cell type used in the RAFT™ Culture System and culture time. Cells embedded in RAFT™ System are evenly...
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