Data Type
Cell Information
(853)
Citations
(4772)
FAQ
(723)
Culture Media
(93)
Category
Primary Cells and Media
(274)
Transfection
(180)
Laboratory Instrumentation
(96)
Endotoxin Detection
(56)
Electrophoreses and Analysis
(55)
Bioassay
(46)
3D Cell Culture
(42)
Live Cell Imaging
(34)
Mycoplasma Detection and Prevention
(30)
Serum-free and Speciality Media
(15)
Classical Media and Reagents
(11)
Cell Lines and Primary Cancer Cells
(9)
Uncategorized
(7)
Cell Services
(3)
Bioprocess Containers
(1)
+ Show All
Research Area
Uncategorized
(175)
Basic Research
(148)
Cancer Research/Cell Biology
(84)
Immunotherapy / Hematology
(72)
Endotoxin Testing
(55)
Stem Cells
(54)
Molecular Biology
(40)
Neurobiology
(38)
Respiratory Research
(32)
Toxicology
(29)
Cardiovascular
(28)
Regenerative medicine
(24)
Dermatology/Tissue Engineering
(21)
Gene Expression
(15)
Drug Discovery
(5)
Gastroenterology
(4)
Parasitology
(3)
+ Show All
723 results sorted by
relevance
alphabetical
newest first
oldest first
I
o
b
s
e
r
v
e
d
h
i
g
h
m
o
r
t
a
l
i
t
y
r
a
t
e
s
i
n
m
E
S
c
e
l
l
s
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
™
w
i
t
h
a
C
r
e
r
e
c
o
m
b
i
n
a
s
e
e
x
p
r
e
s
s
i
o
n
v
e
c
t
o
r
.
H
o
w
c
a
n
I
i
n
c
r
e
a
s
e
t
h
e
v
i
a
b
i
l
i
t
y
?
The High mortality after transfection might be due to the Cre recombinase itself, because the mammalian genome contains pseudo loxP sites (Thyagarajan B et al. (2000) Mammalian genomes contain active recombinase recognition sites. Gene....
C
a
n
L
o
n
z
a
'
s
a
n
t
i
b
i
o
t
i
c
s
b
e
u
s
e
d
i
n
c
l
i
n
i
c
a
l
t
r
i
a
l
s
?
No. They are for research purposes only.
W
h
a
t
i
s
t
h
e
b
a
s
i
c
p
r
i
n
c
i
p
l
e
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
T
e
c
h
n
o
l
o
g
y
?
The Nucleofector™ Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell-type...
W
h
y
i
s
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
T
e
c
h
n
o
l
o
g
y
i
d
e
a
l
f
o
r
p
r
i
m
a
r
y
c
e
l
l
s
a
n
d
d
i
f
f
i
c
u
l
t
-
t
o
-
t
r
a
n
s
f
e
c
t
c
e
l
l
l
i
n
e
s
?
There are several reasons to choose the Nucleofector™ Technology for your gene transfer experiments. One is the direct transfer of DNA to the cell nucleus. This makes gene expression independent from cell division. Therefore, the technique is the...
D
o
y
o
u
h
a
v
e
a
p
r
o
t
o
c
o
l
f
o
r
s
t
i
m
u
l
a
t
i
o
n
o
f
m
o
u
s
e
B
c
e
l
l
s
?
Yes, if you want to transfect mouse B cells, we recommend to stimulate the cells before. The information is also available in the mouse B cell nucleofection protocol. Our R&D has used following combination : RPMI1640 [Lonza; Cat. No....
W
h
a
t
i
s
t
h
e
t
y
p
i
c
a
l
c
e
l
l
d
i
s
t
r
i
b
u
t
i
o
n
f
o
r
p
e
r
i
p
h
e
r
a
l
b
l
o
o
d
m
o
n
o
n
u
c
l
e
a
r
c
e
l
l
s
(
P
B
M
C
)
c
a
t
a
l
o
g
n
u
m
b
e
r
C
C
-
2
7
0
2
?
We don't characterize each lot of these cells. Therefore, we don't make any guarantee regarding cell type distribution.Typically, you would expect to see approximately 60-80% T Cells, 10% monocytes, and the remaining cells would be Beta cells,...
W
h
i
c
h
m
o
b
i
l
i
z
a
t
i
o
n
r
e
g
i
m
e
h
a
s
b
e
e
n
u
s
e
d
f
o
r
t
h
e
C
D
3
4
+
c
e
l
l
s
f
r
o
m
m
o
b
i
l
i
z
e
d
p
e
r
i
p
h
e
r
a
l
b
l
o
o
d
(
4
Y
-
1
0
1
)
?
C
a
n
I
a
l
s
o
h
a
v
e
t
h
e
c
e
l
l
s
f
r
o
m
d
o
n
o
r
s
t
r
e
a
t
e
d
w
i
t
h
a
n
o
t
h
e
r
m
o
b
i
l
i
z
a
t
i
o
n
r
e
g
i
m
e
?
The mobilized peripheral blood used for 4Y-101 is derived from peripheral blood mobilized with Neupogen® 10 mcg/kg/day x 5 days. The cryopreserved cells can be isolated from day 5 and/or day 6 collections. It is possible to request...
D
o
e
s
D
M
S
O
(
e
x
.
b
r
o
u
g
h
t
i
n
w
i
t
h
t
h
e
s
u
b
s
t
r
a
t
e
)
a
f
f
e
c
t
N
u
c
l
e
o
f
e
c
t
i
o
n
?
Most research paper agrees that the upper limit of cell tolerance to DMSO presence in their cell culture media is about 1% final DMSO (this might vary with the cells and media).In the case of the Nucleofection, the DMSO will likely be transfected (at...
C
a
n
I
r
e
p
l
a
c
e
t
h
e
R
A
F
T
c
o
l
l
a
g
e
n
w
i
t
h
a
n
o
t
h
e
r
h
y
d
r
o
g
e
l
t
y
p
e
e
x
.
M
a
t
r
i
g
e
l
?
Complete substitution for collagen type I within the RAFT system (including its absorption step) is not possible, but one can certainly add Matrigel or laminins to the RAFT collagen mixture, up to a certain ratio. The absorption conditions (ex. time)...
W
h
a
t
i
s
t
h
e
C
o
c
o
o
n
®
t
e
c
h
n
o
l
o
g
y
?
The Cocoon® Platform utilizes a single use, disposable cassette to functionally close and automate cell manufacturing. Primarily developed to automated all steps in CAR-T cell manufacturing, the Cocoon® Platform is being used for numerous...
PREVIOUS
PAGE
1
PAGE
2
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
NEXT