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How quickly can I produce a 3D cell culture using the RAFT System?

Using the RAFT™ System, it takes less than 1 hour to create 3D collagen cell cultures – individually or in batches depending on your research requirements.

When should the in-vitro fm be calculated in the course of drug development?

The in-vitro fm can be calculated: - early in the development of the test compound, by using the Silensomes™ HLM of 3 key CYPs involved in the biotransformation of many drugs, CYP3A4 and CYP1A2, and CYP2D6. (Catalog numbers SIL200K, SIL210K, and...

What are the advantages of the RAFT 3D Cell Culture System over coatings such as Matrigel or similar?

Growing cells in the RAFT™ 3D Culture System versus on any other coating including collagen, Matrigel™ or similar, comes to compare 3D to 2D cell culture. You might notice big differences in cells behavior and response. Cell-to-cell or -matrix...

Why is collagen used in the formation of RAFT Cultures?

The RAFT™ System uses type I collagen, the major component of natural extracellular matrix (ECM), as it is the ideal material for forming realistic 3D cell cultures. The patented RAFT™ Process enables a physiologically relevant concentration of...

I have run out of the Master Control Silensomes HLM. How do I get more that will work with my existing stocks of the inhibited Silensomes HLM?

Because the control Silensomes™ HLM must be made in the same production run as the inhibited Silensomes™ HLM, it’s important to compare the batch numbers on each vial. To determine whether the batch runs match, compare the last 4 digits of the lot...

What are the advantages of RAFT 3D Cultures over other 3D hydrogel cultures?

With the compression, the RAFT™ 3D Cell Culture System helsp to obtain high density collagens within a final concentration of 80 mg/ml that resemble to the in vivo tissue collagen density in the same magnitude. Classical hydrogels without compression...

What is special about the RAFT Process?

The RAFT™ Process allows the user to control the process of making 3D cell cultures. The RAFT™ System enables the researcher to determine the cell seeding density in their cultures and supports more complex cell co-culture. Cells can be cultured...

I have a freezer full of HLM already, can you make Silensomes from my existing stock?

Yes, we would initiate a custom Silensomes project with you to evaluate your current stock and create a project to develop the Silensomes™ from it. There is a certain amount of activity loss when performing the inhibition reactions, so you not be...

How well do RAFT Cell Cultures mimic in vivo environment?

Several papers have been published describing the results of using this novel process and a range of methods and analytical approaches have been used which show that the cell cultures produced mimic the cells natural environment.

What is the thickness of the 3D cell cultures produced with the RAFT System?

RAFT™ 3D Cell Cultures made according to the standard protocol have a thickness of approximately 100-120µm – slightly influenced by the cell type used in the RAFT™ Culture System and culture time. Cells embedded in RAFT™ System are evenly...