Data Type
Cell Information
(844)
Citations
(4661)
FAQ
(679)
Culture Media
(93)
Category
Primary Cells and Media
(264)
Transfection
(176)
Laboratory Instrumentation
(68)
Electrophoreses and Analysis
(55)
Bioassay
(48)
3D Cell Culture
(41)
Live Cell Imaging
(34)
Mycoplasma Detection and Prevention
(29)
Endotoxin Detection
(27)
Serum-free and Speciality Media
(11)
Classical Media and Reagents
(10)
Cell Lines and Primary Cancer Cells
(7)
Uncategorized
(7)
+ Show All
Research Area
Uncategorized
(173)
Basic Research
(147)
Cancer Research/Cell Biology
(83)
Immunotherapy / Hematology
(66)
Stem Cells
(53)
Molecular Biology
(40)
Neurobiology
(38)
Respiratory Research
(31)
Toxicology
(28)
Endotoxin Testing
(27)
Cardiovascular
(26)
Regenerative medicine
(23)
Dermatology/Tissue Engineering
(21)
Gene Expression
(13)
Gastroenterology
(4)
Parasitology
(3)
+ Show All
679 results sorted by
relevance
alphabetical
newest first
oldest first
W
h
a
t
i
s
t
h
e
b
a
s
i
c
p
r
i
n
c
i
p
l
e
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
T
e
c
h
n
o
l
o
g
y
?
The Nucleofector™ Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell-type...
D
o
y
o
u
r
e
c
o
m
m
e
n
d
p
o
s
i
t
i
v
e
s
e
l
e
c
t
i
o
n
o
r
d
e
p
l
e
t
i
o
n
f
o
r
t
h
e
p
u
r
i
f
i
c
a
t
i
o
n
o
f
H
u
m
a
n
m
o
n
o
c
y
t
e
s
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
We only recommend the depletion method. For Example, the RosetteSep™ Isolation Kit for human monocytes [Stem Cell Technologies, Cat. No 15028]. The advantage of depletion is that the monocytes are left untouched by antibodies during the process.
W
h
y
i
s
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
T
e
c
h
n
o
l
o
g
y
i
d
e
a
l
f
o
r
p
r
i
m
a
r
y
c
e
l
l
s
a
n
d
d
i
f
f
i
c
u
l
t
-
t
o
-
t
r
a
n
s
f
e
c
t
c
e
l
l
l
i
n
e
s
?
There are several reasons to choose the Nucleofector™ Technology for your gene transfer experiments. One is the direct transfer of DNA to the cell nucleus. This makes gene expression independent from cell division. Therefore, the technique is the...
D
o
e
s
i
t
m
a
t
t
e
r
i
f
t
h
e
P
B
S
u
s
e
d
f
o
r
m
o
n
o
c
y
t
e
e
n
r
i
c
h
m
e
n
t
c
o
n
t
a
i
n
s
c
a
l
c
i
u
m
a
n
d
m
a
g
n
e
s
i
u
m
,
i
f
t
h
e
c
e
l
l
s
s
h
o
u
l
d
b
e
u
s
e
d
l
a
t
e
r
i
n
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
The PBS should be calcium and magnesium free to prevent clumping of cells. We routinely use PBS without calcium and magnesium for example Lonza Cat. No. 17-516F
W
h
a
t
o
p
t
i
m
i
z
a
t
i
o
n
i
s
n
e
c
e
s
s
a
r
y
t
o
g
e
t
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
T
e
c
h
n
o
l
o
g
y
t
o
w
o
r
k
?
None. For each cell type in our product list we offer a cell-type specific solution and thoroughly optimized electrical parameters. These are already pre-programmed in the Nucleofector™ Device. Additionally our optimized protocols give you...
W
h
a
t
c
a
n
I
d
o
w
h
e
n
t
h
e
r
e
i
s
n
o
o
p
t
i
m
i
z
e
d
p
r
o
t
o
c
o
l
a
v
a
i
l
a
b
l
e
f
o
r
m
y
c
e
l
l
o
f
i
n
t
e
r
e
s
t
?
"Lonza is constantly developing new optimized kits and protocols for an increasing number of primary cells and cell lines. In order to obtain the latest information, check our website or contact our Scientific Support Team. We are offering Cell Line...
H
o
w
c
a
n
I
d
e
t
e
r
m
i
n
e
t
h
e
c
o
r
r
e
c
t
c
e
n
t
r
i
f
u
g
a
t
i
o
n
s
p
e
e
d
f
o
r
m
y
p
a
r
t
i
c
u
l
a
r
c
e
n
t
r
i
f
u
g
e
a
n
d
r
o
t
o
r
?
The correct rotor speed can be calculated by measuring the maximum radius of your rotor and entering the information into the table found on the website which can be reached by clicking on the Related Link.
W
h
a
t
i
s
t
h
e
b
e
s
t
w
a
y
t
o
e
s
t
a
b
l
i
s
h
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
T
e
c
h
n
o
l
o
g
y
i
n
m
y
l
a
b
?
We strongly recommend establishing the Nucleofector™ Technology with the positive control vector pmaxGFP™ provided in our kits. pmaxGFP encodes the green fluorescent protein (GFP) from Copepod Pontellina sp. Just like eGFP expressing cells, maxGFP™...
I
s
t
h
e
r
e
a
s
p
e
c
i
a
l
r
e
c
o
m
m
e
n
d
a
t
i
o
n
o
n
H
u
m
a
n
T
c
e
l
l
e
n
r
i
c
h
m
e
n
t
b
e
f
o
r
e
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
For Nucleofection™ it is preferable to use Human T cell populations enriched by magnetic separation. For example, MACS™ Microbeads (Miltenyi) or by a rosetting method. For magnetic separation we recommend negative selection or detaching beads after...
A
r
e
t
h
e
r
e
a
n
y
r
e
c
o
m
m
e
n
d
a
t
i
o
n
s
t
o
a
v
o
i
d
e
x
c
e
s
s
i
v
e
m
o
r
t
a
l
i
t
y
d
u
r
i
n
g
T
c
e
l
l
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
Try to keep the DNA amount for Nucleofection™ quite low (e.g. 1 µg plasmid DNA per 100 µl reaction) since higher DNA amounts might cause increasing toxicity and mortality. It is also important to use highly purified DNA, e.g. by using Endofree™...
PREVIOUS
PAGE
1
PAGE
2
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
NEXT