Q:

Is it possible to use frozen primary CD34+ hematopoietic stem cells for Nucleofection™?

A:

Yes. For cryopreserved PBMCs or enriched CD34 populations, we recommend culturing the thawed cells 1-2 hours in culture medium before Nucleofection™. Any further enrichment procedure after thawing is not recommended. Viability of frozen nucleofected cells might be lower than that of fresh ones.

Related Cells:
Categories:
Primary Cells and Media
Transfection
Research Areas:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Stem Cells
Basic Research
Molecular Biology
Regenerative medicine