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Do you offer on-site maintencance services for the 4D-Nucleofector Core, X, Y or LV Unit? 

No, currently we can only offer in-house maintenance services, but we are working on a process for on-site services.

Do you have any instructions for performing a trypan blue stain?

Yes. Please click on the link under related literature.

 What are the main differences between Earle's BSS and Hank's BSS? 

A balanced salt solution (BSS) is a solution made to a physiological pH and salt concentration.  Solutions most commonly include sodium, potassium, calcium, magnesium, and chloride.   Balanced salt solutions are used for washing...

What is HEPES? 

HEPES ( 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent ; one of the twelve Good's buffers . HEPES is widely used in cell culture , largely because it is better at maintaining...

I am using the acute leukemia HL-60 cell line for CAR-T cells experiments. Do you have a transfection protocol for genome editing?

The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors). Transfection protocols can be...

Do you have any tips for the culture of primary cells or cell line prior to transfection?

Yes. Please see our Techincal Reference Guide which contains many helpful tips.

How do you recommend that we isolate splenic lymphocytes from mouse spleens for Nucleofection? Is erythrocyte lysis required?

For the preparation of mouse spleen cells we recommend cutting the spleen once and passing the tissue through a 100µM cell strainer or steel mesh using a plunger. The cells are flushed into a petri dish containing PBS. In order to remove fat, cell...

Do you recommend positive selection or depletion for the purification of Human monocytes for Nucleofection?

We only recommend the depletion method. For Example, the RosetteSep™ Isolation Kit for human monocytes [Stem Cell Technologies, Cat. No 15028]. The advantage of depletion is that the monocytes are left untouched by antibodies during the process.

Does it matter if the PBS used for monocyte enrichment contains calcium and magnesium, if the cells should be used later in Nucleofection ?

The PBS should be calcium and magnesium free to prevent clumping of cells. We routinely use PBS without calcium and magnesium for example Lonza Cat. No. 17-516F

How can I determine the correct centrifugation speed for my particular centrifuge and rotor?

The correct rotor speed can be calculated by measuring the maximum radius of your rotor and entering the information into the table found on the website which can be reached by clicking on the Related Link.
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