Data Type
Cell Information
(858)
Citations
(4968)
FAQ
(515)
Culture Media
(61)
Category
Primary Cells and Media
(200)
Transfection
(170)
Laboratory Instrumentation
(73)
Endotoxin Detection
(54)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
3D Cell Culture
(6)
Classical Media and Reagents
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(135)
Cancer Research/Cell Biology
(77)
Immunotherapy / Hematology
(63)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Toxicology
(22)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(14)
Drug Discovery
(7)
Parasitology
(2)
Gastroenterology
(1)
+ Show All
515 results sorted by
relevance
alphabetical
newest first
oldest first
W
h
a
t
i
s
t
h
e
b
a
s
i
c
p
r
i
n
c
i
p
l
e
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
T
e
c
h
n
o
l
o
g
y
?
The Nucleofector® Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell-type...
W
h
y
i
s
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
T
e
c
h
n
o
l
o
g
y
i
d
e
a
l
f
o
r
p
r
i
m
a
r
y
c
e
l
l
s
a
n
d
d
i
f
f
i
c
u
l
t
-
t
o
-
t
r
a
n
s
f
e
c
t
c
e
l
l
l
i
n
e
s
?
There are several reasons to choose the Nucleofector® Technology for your gene transfer experiments. One is the direct transfer of DNA to the cell nucleus. This makes gene expression independent from cell division. Therefore, the technique is the...
W
h
a
t
o
p
t
i
m
i
z
a
t
i
o
n
i
s
n
e
c
e
s
s
a
r
y
t
o
g
e
t
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
T
e
c
h
n
o
l
o
g
y
t
o
w
o
r
k
?
None. For each cell type in our product list we offer a cell-type specific solution and thoroughly optimized electrical parameters. These are already pre-programmed in the Nucleofector® Device. Additionally our optimized protocols give you...
W
h
a
t
c
a
n
I
d
o
i
f
t
h
e
r
e
i
s
n
o
N
u
c
l
e
o
f
e
c
t
i
o
n
®
o
p
t
i
m
i
z
e
d
p
r
o
t
o
c
o
l
a
v
a
i
l
a
b
l
e
f
o
r
m
y
c
e
l
l
o
f
i
n
t
e
r
e
s
t
?
Lonza is constantly developing new optimized kits and protocols for an increasing number of primary cells and cell lines. In order to obtain the latest information, check our website or contact our Scientific Support Team.We are offering Primary Cell...
I
s
t
h
e
r
e
a
s
p
e
c
i
a
l
r
e
c
o
m
m
e
n
d
a
t
i
o
n
o
n
H
u
m
a
n
T
c
e
l
l
e
n
r
i
c
h
m
e
n
t
b
e
f
o
r
e
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
For Nucleofectio® it is preferable to use Human T cell populations enriched by magnetic separation. For example, MACS™ Microbeads (Miltenyi) or by a rosetting method. For magnetic separation we recommend negative selection or detaching beads...
A
r
e
t
h
e
r
e
a
n
y
r
e
c
o
m
m
e
n
d
a
t
i
o
n
s
t
o
a
v
o
i
d
e
x
c
e
s
s
i
v
e
m
o
r
t
a
l
i
t
y
d
u
r
i
n
g
T
c
e
l
l
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
Try to keep the DNA amount for Nucleofection® quite low (e.g. 1 µg plasmid DNA per 100 µl reaction) since higher DNA amounts might cause increasing toxicity and mortality. It is also important to use highly purified DNA, e.g. by using Endofree™...
H
o
w
c
a
n
I
i
n
c
r
e
a
s
e
c
e
l
l
v
i
a
b
i
l
i
t
y
o
f
D
e
n
d
r
i
t
i
c
c
e
l
l
s
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
DNA amount and quality are very critical for Nucleofection® of Dendritic Cells (recommended 0.5-1 µg; maximal 2 µg). LPS (lipopolysaccharides) have a strong negative effect on cell viability. Please make sure that all components you use for dendritic...
I
w
a
n
t
t
o
s
t
i
m
u
l
a
t
e
H
u
m
a
n
T
c
e
l
l
s
p
o
s
t
N
u
c
l
e
o
f
e
c
t
i
o
n
®
.
A
r
e
t
h
e
r
e
a
n
y
p
r
e
c
a
u
t
i
o
n
s
t
o
k
e
e
p
i
n
m
i
n
d
?
After Nucleofection®, wait at least four hours before stimulation. Stimulating immediately after Nucleofection® may lead to increased cell mortality and poor activation of cells. For more details on the stimulation of Human T cells post...
A
r
e
y
o
u
r
N
u
c
l
e
o
f
e
c
t
o
r
®
S
o
l
u
t
i
o
n
s
c
h
e
c
k
e
d
f
o
r
R
N
a
s
e
a
c
t
i
v
i
t
y
d
u
r
i
n
g
y
o
u
r
Q
C
p
r
o
c
e
s
s
?
Yes. We do test the Solutions with a real time RT-PCR based assay. This assay basically measures the quantity of an RNA template after incubation in our solution. In case there is RNAse contamination the template would be digested which would be...
W
h
a
t
k
i
n
d
o
f
b
u
f
f
e
r
d
o
y
o
u
r
e
c
o
m
m
e
n
d
f
o
r
r
e
s
u
s
p
e
n
d
i
n
g
m
y
s
i
R
N
A
?
Please use the buffer that is recommended by your siRNA manufacturer.
PREVIOUS
PAGE
1
PAGE
2
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
NEXT