Data Type
Cell Information
(856)
Citations
(4853)
FAQ
(712)
Culture Media
(92)
Category
Primary Cells and Media
(277)
Transfection
(197)
Laboratory Instrumentation
(78)
Endotoxin Detection
(57)
Electrophoreses and Analysis
(56)
Bioassay
(48)
3D Cell Culture
(43)
Mycoplasma Detection and Prevention
(34)
Serum-free and Speciality Media
(15)
Classical Media and Reagents
(11)
Cell Lines and Primary Cancer Cells
(9)
Uncategorized
(7)
Live Cell Imaging
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(1)
Protozoa
(1)
+ Show All
Research Area
Uncategorized
(174)
Basic Research
(129)
Cancer Research/Cell Biology
(85)
Immunotherapy / Hematology
(73)
Endotoxin Testing
(55)
Stem Cells
(53)
Molecular Biology
(40)
Neurobiology
(39)
Respiratory Research
(33)
Toxicology
(33)
Cardiovascular
(28)
Regenerative medicine
(25)
Dermatology/Tissue Engineering
(21)
Gene Expression
(16)
Drug Discovery
(7)
Gastroenterology
(4)
Parasitology
(3)
+ Show All
712 results sorted by
relevance
alphabetical
newest first
oldest first
W
h
a
t
i
s
t
h
e
p
r
o
o
f
t
h
a
t
D
N
A
e
n
t
e
r
s
t
h
e
n
u
c
l
e
u
s
d
u
r
i
n
g
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
There are two facts that prove that the DNA is directly transported into the nucleus: 1.) Using the Nucleofector™ Technology, transfection of non-dividing cells is possible. With conventional non-viral transfection methods cell division is a...
H
o
w
c
a
n
I
r
e
m
o
v
e
t
h
e
c
e
l
l
s
f
r
o
m
t
h
e
1
0
0
u
L
N
u
c
l
e
o
c
u
v
e
t
t
e
™
V
e
s
s
e
l
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
I
s
t
h
e
r
e
a
n
y
a
l
t
e
r
n
a
t
i
v
e
?
We recommend using the plastic single use pipettes provided in the kits. These pipettes prevent any damage to the cells and ensure a good cell recovery.
W
h
a
t
m
e
t
h
o
d
c
a
n
I
u
s
e
t
o
f
i
x
m
y
H
L
-
6
0
c
e
l
l
s
a
s
s
h
o
w
n
i
n
y
o
u
r
o
p
t
i
m
i
z
e
d
p
r
o
t
o
c
o
l
?
The cells which are shown in the HL-60 optimized protocol are living cells and not fixed cells. However a good method for fixation of GFP is to use 3.5%-4% Paraformaldehyde in PBS. Please keep in mind that the fixation method is dependant on what you...
I
s
y
o
u
r
p
m
a
x
G
F
P
c
o
n
t
r
o
l
v
e
c
t
o
r
s
u
p
p
l
i
e
d
w
i
t
h
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
K
i
t
s
s
u
i
t
a
b
l
e
f
o
r
s
t
a
b
l
e
e
x
p
r
e
s
s
i
o
n
?
No. This vector is only supplied in our Nucleofector™ Kits as a positive control and cannot be used for selection because it does not contain a mammalian selection marker. The only resistance gene expressed by this vector is kanamycin which is only...
C
a
n
I
i
n
t
e
g
r
a
t
e
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
i
n
t
o
a
l
i
q
u
i
d
h
a
n
d
l
i
n
g
s
y
s
t
e
m
?
Yes. The 96-well Shuttle™ Device is designed in such a way that it is addressable by robot grippers. Furthermore, the software contains the required interface to be connected with the LHS software. However, the practical implementation for each...
C
a
n
I
u
s
e
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
2
b
S
o
l
u
t
i
o
n
s
a
n
d
O
p
t
i
m
i
z
e
d
P
r
o
t
o
c
o
l
s
w
i
t
h
t
h
e
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
™
X
-
U
n
i
t
o
r
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
?
No. The 4D-Nucleofector and 96-well Shuttle™ System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle™ Solutions are available for primary cells. For cell lines, three different 96-well...
D
o
y
o
u
h
a
v
e
a
n
y
r
e
c
o
m
m
e
n
d
e
d
m
e
t
h
o
d
s
f
o
r
d
e
t
a
c
h
i
n
g
h
u
m
a
n
m
o
n
o
c
y
t
e
s
f
r
o
m
t
h
e
p
l
a
t
e
f
o
r
a
s
s
a
y
i
n
g
o
r
b
e
f
o
r
e
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
We have had good results by incubating the cells in ice cold PBS for 10 minutes and then rinsing the plates. Alternatively, the cells can be detached without a medium change by gently pipetting the cell suspension up and down. Our experience has also...
C
a
n
I
u
s
e
a
N
u
c
l
e
o
f
e
c
t
o
r
™
I
o
r
N
u
c
l
e
o
f
e
c
t
o
r
™
2
b
D
e
v
i
c
e
t
o
g
e
t
h
e
r
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
?
No, the 96-well Shuttle™ only works in conjunction with the Nucleofector™ IIs Device or our 4D-Nucleofector™ Device.
I
s
i
t
p
o
s
s
i
b
l
e
t
o
u
s
e
d
i
f
f
e
r
e
n
t
s
u
b
s
t
r
a
t
e
s
(
e
.
g
.
s
i
R
N
A
d
u
p
l
e
x
e
s
)
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
?
Yes. Transfection can be done with any substrate AND under exactly the same conditions. For example, the same conditions (96-well Shuttle™ Solution and program) are used for siRNA transfection or shRNA vectors. This greatly facilitates the...
W
h
a
t
i
s
t
h
e
b
a
s
i
c
p
r
i
n
c
i
p
l
e
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
T
e
c
h
n
o
l
o
g
y
?
The Nucleofector™ Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell-type...
PREVIOUS
PAGE
1
PAGE
2
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
NEXT