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Can I download the software for the FlashGel Camera from your website?  

Please check the serial number of your camera. For cameras shipped after July 2012 ( serial # >2012 ), version 3.2 of the software is available for download here:  https://lonza.picturepark.com/Go/7BAf8Tgu/D Compatible with Windows®...

Do you offer on-site maintencance services for the 4D-Nucleofector Core, X, Y or LV Unit? 

No, currently we can only offer in-house maintenance services, but we are working on a process for on-site services.

Do you have any instructions for performing a trypan blue stain?

Yes. Please click on the link under related literature.

 What are the main differences between Earle's BSS and Hank's BSS? 

A balanced salt solution (BSS) is a solution made to a physiological pH and salt concentration.  Solutions most commonly include sodium, potassium, calcium, magnesium, and chloride.   Balanced salt solutions are used for washing...

What is HEPES? 

HEPES ( 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent ; one of the twelve Good's buffers . HEPES is widely used in cell culture , largely because it is better at maintaining...

I am using the acute leukemia HL-60 cell line for CAR-T cells experiments. Do you have a transfection protocol for genome editing?

The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors). Transfection protocols can be...

Do you have any tips for the culture of primary cells or cell line prior to transfection?

Yes. Please see our Techincal Reference Guide which contains many helpful tips.

What are the technical specifications for the CytoSMART 2 System?

Field of View: 2.4 x 1.4 mm2 Magnifiction: 10X Image Resolution: 1280 x 720 pixels (converted to 640 x 360 [72 dpi] on the CytoSMART™ Project Page Exported formats: JPEG, CSV, AVI Light source: Green LED Camera: 5 MP CMOS Diplay: Remote 10"...


How do you recommend that we isolate splenic lymphocytes from mouse spleens for Nucleofection? Is erythrocyte lysis required?

For the preparation of mouse spleen cells we recommend cutting the spleen once and passing the tissue through a 100µM cell strainer or steel mesh using a plunger. The cells are flushed into a petri dish containing PBS. In order to remove fat, cell...
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