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What is the component labeled "MCGS" in the mesenchymal stem cell growth and differentiation media?

Mesenchymal Cell Growth Supplement (MCGS) is 100% fetal bovine serum prescreened to neither induce nor prevent differentiation of human bone marrow derived stem cells PT-2501

How would you further cultivate the cells after the formation of a RAFT Cellular structure?

For the 96 and 24 well RAFT™ Kit format, you simply put the media on top of the RAFT structure laying already in the well, and cultivate the cells, as you classically do in 2 dimensions. For high cell number in RAFT™ Cultures you might use twice more...

What is the difference between the various mesenchymal stem cell growth and differentiation basal media?

The basal media portions of the MSCGM growth and differentiation media are exactly the same except for the volume and the concentration of glucose. The glucose concentration in the growth (PT-3238) and osteogenic differentiation (PT-3924) basal media...

What is the RAFT 3D Cell Culture System?

RAFT™ System is a superior 3D cell culture system to enable the production of collagen-based cultures, simply and reliably. It combines a novel patented process with specially-designed RAFT™ Absorbers and reagents in combination with standard cell...

When a RAFT 3D Culture is subjected to an immunofluorescence labeling, should one expect a high background coming from antibody cross reactivity with the matrix?

Our Technical Note “Immunofluorescence Visualization of Fibroblast or Cancer Cells in RAFT™ 3D Cell Cultures” shows no immunofluorescence cross reactivity between the used antibodies and the rat collagen matrix, even with secondary antibodies...

How does the RAFT System produce 3D cell cultures?

A mix of collagen, cells and other reagents is pipetted into the wells of a plate and incubated for 15 minutes to form a collagen hydrogel. Subsequently the biocompatible RAFT™ Absorbers are placed on top of the gels and over a 15 minute period a...

Whats the difference in the immunofluorescence protocol for RAFT 3D Cultures and for 2 dimensional cell cultures?

First of all, immunofluorescence labeling works for RAFT™ 3D Cultures like for 2D cultures. The labeling protocol is basically the same. When visualizing structures within cellular aggregates such as tumoroids, a more intensive permeabilization step...

In which type of cell culture vessels are the RAFT 3D Cell Cultures produced?

RAFT™ can produce the 3D cell cultures in each well of standard 96-well and 24-well cell culture plates as well as in 24-well cell culture inserts (6,5mm diameter, please contact Lonza Scientific Support for a list of RAFT™ compatible cell culture...

How does the in-vitro fm data predict the in-vivo fm value?

The Silensomes™ HLM do not need to incorporate a Relative Activity Factor and therefore directly predict the CYP contribution to drug metabolism. In-vitro fm values calculated using Silensomes™ HLM are directly correlated to in-vivo fm values using...

Is the RAFT 3D Cell Culture permeable to large molecules like antibodies, drugs etc?

Although the RAFT™ Process concentrates the collagen matrix to a physiologically relevant concentration, the matrix itself does not act as a barrier to molecules as big as antibodies (150kDa), since no intensive permeabilisation steps were necessary...
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