Q:

Why do you have different Optimized Protocols for the Nucleofection™ of unstimulated and stimulated Human T cells?

A:

We found that the stimulation of T cells not only changes the cells' function but also significantly alters the requirements for successful Nucleofection™. That's why Lonza developed two Optimized Protocols for transfection giving different electrical parameters for stimulated and unstimulated T cells. In addition, the protocol is optimised for 5-6d stimulated T cells. If you use allostimulated or long term stimulated T cells like LAK cells, please contact our Scientific Support Team for assistance.

Categories:
Transfection
Research Areas:
Immunotherapy / Hematology
Basic Research