The Nucleofector™ Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell-type specific solutions. With the Nucleofector™ Technology the DNA is transported directly into the nucleus. Also with other substrates, such as siRNA or miRNA, Nucleofector™ Technology offers highly efficient and robust transfection with high cell viability.