What is the proof that DNA enters the nucleus during Nucleofection™?


There are two facts that prove that the DNA is directly transported into the nucleus: 1.) Using the Nucleofector™ Technology, transfection of non-dividing cells is possible. With conventional non-viral transfection methods cell division is a prerequisite for DNA entry into the nucleus. Until now, the only method to transfect non-dividing cells was by using a viral vector system. 2.) In nucleofected cells gene expression can be detected shortly after Nucleofection™. In cell lines, the transfected gene can usually be analyzed after just 2-4 hours. Please see the figures on page 4 of our Nucleofector™ Technology Brochure.