Q:

Is there a special recommendation on Human T cell enrichment before Nucleofection®?
A:

For Nucleofectio® it is preferable to use Human T cell populations enriched by magnetic separation. For example, MACS™ Microbeads (Miltenyi) or by a rosetting method. For magnetic separation we recommend negative selection or detaching beads after enrichment. Do not apply protocols using hypo-osmolar buffers. This may lead to high cell mortality after Nucleofectio®.
Related Cells:
T cell, human peripheral blood unstim. ; T cell, human cord blood unstim.
Categories:
Transfection
Research Areas:
Immunotherapy / Hematology

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