Q:

I want to stimulate Human T cells post Nucleofection™. Are there any precautions to keep in mind?

A:

After Nucleofection™, wait at least four hours before stimulation. Stimulating immediately after Nucleofection™ may lead to increased cell mortality and poor activation of cells. For more details on the stimulation of Human T cells post Nucleofection™, please see in the Optimized Protocol.

Categories:
Transfection
Research Areas:
Immunotherapy / Hematology