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The 4D-Nucleofector® X Unit offers flexible throughput from 1 to 16 samples. When working with 100 µl single Nucleocuvettes® you can process two vessels in parallel. However subsequent handling of more than 2 vessels is eneased via the option to...
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They are embryonic. The rats are E18 or E19 and the mice are E14 or E15.
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In contrast to the Nucleofector® I, II and 2b System which comes with individual kits for each primary cell type, the 4D-Nucleofector® System only requires 5 primary cell kits covering a broad range of primary cells. There are cell-type...
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The rats are Sprague-Dawley.
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With conductive polymer electrodes the release of metal ions into the cell suspension is avoided. Conductive polymer electrode Nucloecuvette® Vessels are used in the 4D-Nucleofector® X-Unit and 96-well Unit?
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The cells can remain viable in culture for about 28 days.
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No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...
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No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware
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We do not recommend reusing the conductive polymer cuvettes as performance decreases when cuvettes are used more than once.
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The Rat Neonatal Dorsal Root Ganglion (DRG) Neurons (R-DRG-505) are isolated from neonatal, about 2-3 days old.The Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons (R-eDRG-515) are isolated from embryonic rats (E14-15).
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