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For optimal assay conditions, reconstituted reagent and substrate should be used fresh within 2 hours after reconstitution.During this time they can be kept at room temperature.For the MycoAlert® Kit: Unused, reconstituted components can be aliquoted...
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The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant as sample.Smaller initial aliquots of media may be taken to start with if necessary.
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Error 8 occurs when there has been an arc within the cuvette during application of the electrical program. The pulse application started but was interrupted because too much current was detected. Efficiency may be lower than expected due to the...
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The positive control should be always included as reference for a positive signal. It serves as control for the performance of the MycoAlert® Assay and the MycoAlert® PLUS Assay and the luminescence measurement.
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No, the MycoAlert® Mycoplasma Detection Assay (as well as the MycoAlert® PLUS Mycoplasma Detection Assay) is not quantitative; it has been designed to give a simple Yes or No answer, pertaining to the presence of Mycoplasma.
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This message means that the system cut off the pulse before it was completed (usually this is because the conductivity of the solution is at the low end of the normal range). The pulse was applied; it is just going to be a little weaker than...
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It is highly recommended that the samples are spun down prior to testing. If cells are present in the assay they will contribute to the initial reading producing a higher background. This in turn can decrease the difference between the background and...
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In the back of the respective manuals, there is a chart giving a brief description of the error codes. If more information is needed or an error code is recurrent, it is best to contact Scientific Support for advice to determine if there is a...
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Please follow the protocol exactly as written – add reagent, wait 5 min and read, add substrate, wait 10 minutes and read again.
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?
No. The MycoAlert® Assay is sold for Research Use Only.
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