For optimal assay conditions, reconstituted reagent and substrate should be used fresh within 2 hours after reconstitution.

During this time they can be kept at room temperature.

For the MycoAlert® Kit: Unused, reconstituted components can be aliquoted and stored at -20°C up to six months. When using a frost-free freezer, be aware that temperature does not keep -20°C constantly. For improved stability we would recommend storage at -80°C. Once thawed, reagent and/or substrate must not be refrozen and should be allowed to reach room temperature before use without the aid of artificial heat. They should be equilibrated to room temperature for 15-20 min before use. - Avoid multiple cooling/freezing and heating cycles.

For the MycoAlert® PLUS Kit: - For long-term storage, reconstituted reagent and substrate can be aliquoted and stored at -80°C for up to six months. Once thawed, reagent and/or substrate must not be refrozen and should be allowed to reach room temperature before use without the aid of artificial heat. They should be equilibrated to room temperature for 15-20 min before use. - Avoid multiple cooling/freezing and heating cycles.