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Do you have a protocol for the Nucleofection™ of Plasmodium berghei?

Yes. You can use our new Basic Parasite Nucleofector™ Starter kit for parasitic protozoa. The Basic Parasite Nucleofector Starter Kit (Cat.No. VMI-1001) should help you to determine the optimal program and Nucleofector™ Solution for your...

What are the critical steps for successful Nucleofection™ of monocytic cell lines like THP-1, HL60 and U937?

To achieve optimal results, we strongly recommend following the Optimized Protocols in regard to cell culture conditions (medium, splitting cycle, density before Nucleofection),DNA amount and purity. Please also be sure not to exceed 90xg when...

Which reporter gene should I choose for my reporter gene experiment?

In general, all reporter genes can be used. For some applications (e.g. reporter gene experiments in various primary cells, experiments with late analysis time point due to starving and /or stimulation) a reporter gene with a longer half life than...

Do you recommend a particular supplier for siRNA duplexes for use in Nucleofection™ ?

For siRNA applications using the Nucleofector technology we recommend siRNA duplexes from Dharmacon, part of Thermo Fischer Scientific.

Do you have a protocol for the Nucleofection™ of Plasmodium yoelii?

Yes. You can use our new Basic Parasite Nucleofector™ Starter kit for parasitic protozoa. The Basic Parasite Nucleofector Starter Kit (Cat.No. VMI-1001) should help you to determine the optimal program and Nucleofector Solution for your...

What is the maximum amount of DNA I can use in my Nucleofection™ Experiment?

The overall amount of 5 µg should not be exceeded. Look in the cell specific Optimized Protocol (OP) for the recommended total DNA amount of the cell type of interest.

Can I use the protocol for MCF7 cells for other breast cancer cell lines?

No. Often times closely related cell types will require completely different conditions for Nucleofection™. Therefore, you may want to consider performing a full optimization by using the Cell Line Optimization Kit. Please click on the related link...

Do you have a protocol for Nucleofection™ of parasites?

Yes. You can use our Basic Parasite Nucleofector™ Starter kit for parasitic protozoa. The Basic Parasite Nucleofector Starter Kit (Cat.No. VMI-1001) should help you to determine the optimal program and Nucleofector Solution for your...

When nucleofecting cancer cell lines, do I need to be concerned about passage number?

For the most efficient gene transfer, we recommend using cells that are in logarithmic growth phase and at a passage number of 10-15 (from the time of thaw). This is because some cell lines differentiate and change their features after many...

When should I begin looking for the Luciferase signal after Nucleofection™?

The time course of plasmid expression after Nucleofection™ might be different from the time course seen with other transfection methods. We recommend looking at different time points and start with the first time point as early as 4 to 6 hours post...

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