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Bone Marrow: provides a hematopoietic microenvironment and the highest number of different donors.Cord Blood: more naive cells useful in transplant and stem cell research.Fetal Liver: best for developmental studies and they are highly proliferative....
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In vivo, bronchial epithelial cells differentiate along an abnormal squamous pathway under conditions of retinoid deficiency. The squamous phenotype is characterized by the induction of specific markers such as keratin 13, and the enzymes...
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Mycoplasmas belong to the family Mollicutes, which includes Acholeplasma, Ureaplasma and other species.However the term "Mycoplasma" is most often used as a "cover-all".More than 180 species have been identified of which 20 distinct Mycoplasma...
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A conservative estimate states that between 15-35% of all continuous cell cultures are contaminated with mycoplasma (Drexler HG, Uphof CC; 2002), some estimates are even higher (up to 80 % in some countries (Koshimizu K, Kotani H; 1981).
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The cryopreservation medium is: 85% MSCBM 10% DMSO 5% Human Serum Albumin (w/v)
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For performing the MycoAlert® Assay in theory every luminometer is suitable as long as a specific sensitivity is given. Please find attached our list with luminometers we know to be suited to run the MycoAlert® and MycoAlert® Plus Mycoplasma...
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The original attenuation of the IRES sequence was performed to allow for a greater difference between the expression levels of the upstream gene of interest and the downstream reporter gene. If the downstream reporter gene, the product of less than...
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The MycoAlert® Assay Control Set (LT07-518) is available separately and includes a lyophilized positive control (1 ml) and assay buffer (2 ml) for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls...
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CD34+ cells make up about 2% of the population in bone marrow mononuclear cells.
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The mycoplasma specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in cell culture). 95% of all...
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