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There is evidence to suggest that mycoplasma can survive in the absence of cells, some may even proliferate, but as a rule they are much more viable if cells are present. We have demonstrated that the MycoAlert™ Kit will detect mycoplasma...
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For optimal assay conditions, reconstituted reagent and substrate should be used fresh within 2 hours after reconstitution. During this time they can be kept at room temperature. For the MycoAlert™ Kit : Unused, reconstituted components can be...
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That will depend on the type of medium used. If you are using our MSCGM BulletKit PT-3001, we do not recommend using a matrix with the cells as it might induce differentiation and make the cells difficult to detach from the culture vessel. If you are...
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The MycoAlert™ Assay requires the use of cell free supernatant only! Ideally you should sample your cell cultures and test immediately. If needed you can store the supernatant at room temperature for 8 hours, 2°C-8°C for 1 week. The MycoAlert™...
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The shelf-life of the MSCGM Mesenchymal Stem Cells Growth Medium (PT-3001) once the SingleQuots are added is 30 days. For the differentiation kits (PT-3002, PT-3003, PT-3004), its 14 days. This is the shelf life after the growth factors have been...
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The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.
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Bone Marrow: provides a hematopoietic microenvironment and the highest number of different donors Cord Blood: more naive cells useful in transplant and stem cell research Fetal Liver: best for developmental studies and they are highly...
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No, the MycoAlert™ Assay is not quantitative; it has been designed to give a simple Yes or No answer, pertaining to the presence of Mycoplasma.
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85% MSCBM 10% DMSO 5% Human Serum Albumin (w/v)
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It is highly recommended that the samples are spun down prior to testing. If cells are present in the assay they will contribute to the initial reading producing a higher background. This in turn can decrease the difference between the background and...
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