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How many different primary cell kits are available for the 4D-Nucleofector® System?

In contrast to the Nucleofector® I, II and 2b System which comes with individual kits for each primary cell type, the 4D-Nucleofector® System only requires 5 primary cell kits covering a broad range of primary cells. There are cell-type...

Where are Lonza’s Small Airway Epithelial cells isolated from?

Lonza’s Small Airway Epithelial cells (both normal and diseased) are isolated from the distal portion of the lung in the 1 mm bronchiole area. These cells are isolated from airway tubules 1mm in diameter and smaller. Anything with a diameter greater...

What is the difference between Lonza’s ALI screened airway epithelial cells and the non-screened cells?

All of Lonza’s airway epithelial cells receive standard quality control testing in monolayer culture. After cells have passed Lonza’s initial monolayer quality control testing, a few lots receive additional quality control testing to monitor...

What is the advantage of using conductive polymer as electrode material for the 4D-Nucleofector® X-Unit and 96-well Unit?

With conductive polymer electrodes the release of metal ions into the cell suspension is avoided. Conductive polymer electrode Nucloecuvette® Vessels are used in the 4D-Nucleofector® X-Unit and 96-well Unit?

Can I use the settings recommended for the Nucleofector® I/II or 2b System with my 4D-Nucleofector® System?

No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...

Do you have any recommendation how to coat glass cover slips for neuron culture, e.g. for use with the Y unit or for post Nucleofection® culture in general?

Preparation of coverslips: At Lonza we used coverslips from Menzel (part of Thermo Scientific). We think that the coverslips have such a good quality that they don’t have to be cleaned prior to use.If you want to use other coverslips or prefer...

Can I reuse the 100µl Nucleocuvette® Vessel?

We do not recommend reusing the conductive polymer cuvettes as performance decreases when cuvettes are used more than once.

Can you use the AdipoRed™ Assay on cells other than preadipocytes?

Yes, the AdipoRed™ Assay Reagent can be used on many other cell types that accumulate triglycerides. It may have to be optimized for specific applications depending on the lipid that is stained.

How many cells do I have to use for each well of the 16-well Nucleocuvette® Strip?

This depends on the cell type you are working with. In our Optimized Protocols the cell numbers range from 2.5 x 10^4 cells (mouse DC) up to 1x 10^6 cells (human T cells). Detailed information is provided in our 4D-Nucleofector® Optimized Protocols.

Do I have to apply the same program to each well of the 16-well Nucleocuvette®?

No, each well can be addressed separately. You may apply up to 16 different programs to one 16-well Nucleocuvette® Strip.
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