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515 results sorted by
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After withdrawing the dipping electrode array from the plate you can wipe off residual liquid with a tissue. Alternatively, you can wash used electrodes with ethanol which won't damage the electrodes. To avoid cross-contamination of non-used...
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We recommend allowing the transfected hepatocytes to recover in Plating medium for 72 hours post-Nucleofection®.For induction studies (e.g. CYP3A4), the Plating medium is replaced with maintenance medium (William's E without FCS and EGF) and...
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Please find attached a list with all plate types that have been checked for compatibility so far. If your plate type is not on this list, please contact our Scientific Support Team we can investigate compatibility.
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Human hepatocytes do not like to be cultured and transfected at low densities. Therefore, using less than 100,000 cells per Nucleofection® is not recommended.
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Mycoplasma contamination can: > Inhibit cell metabolism > Cause cell death > Induce morphological alterations > Alter DNA, RNA, and protein synthesis > Induce chromosomal aberrations > Cause increased sensitivity to inducers of...
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We recommend using the 20-minute MycoAlert® Mycoplasma Detection Kit from Lonza. The simple four-step MycoAlert® Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid...
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Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells. The MycoZap™ Reagent eliminates...
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Yes. Our technical guideline "Cell Counting and Determination of Viability via Hemocytometer" can be found as associated pdf file in the link below.
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Cells might have been disturbed or ran dry during pipetting steps. Please perform liquid removal and additions well-by-well. Avoid leaving neurons without any liquid coverage (medium or Nucleofector® Solution). To keep a small liquid film on the...
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8
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Error 8 occurs when there has been an arc within the cuvette during application of the electrical program. The pulse application started but was interrupted because too much current was detected. Efficiency may be lower than expected due to the...
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