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How long will your Clonetics™ rat DRG's (dorsal root ganglion cells) remain viable in culture?

The cells can remain viable in culture for about 28 days.

I thawed and plated my ampoule of Clonetics™ Cells yesterday and most of the cells are floating. What happened?

Clonetics™ Cells will not be confluent on day one. After 24 hours, you will see few attached cells per field under the microscope. Aspirate off the media and the floating cells and replace with fresh, pre-warmed media. Those attached cells will begin...

Do the Clonetics™ Rat and Mouse Astrocytes require an extracellular matrix for plating?

No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware

What are the benefits of using agarose for protein gel electrophoresis?

Protein electrophoresis in agarose gels is an alternative approach to using polyacrylamide gels and provides several benefits: - Separate high molecular weight proteins (>600 kDa)- Easy to prepare and handle- Efficient recovery of proteins-...

Can the ProSieve™ Molecular Weight Markers be used on native gels?

No, they are suitable for use on denaturing gels only.

I've had my Clonetics™ Cells in culture for a few months, they're no longer growing and don't look like healthy cells, what's wrong?

All Clonetics™ Cells are primary cells with a finite life span. Most Clonetics™ Cells are guaranteed for 10 – 15 additional population doublings, which equates to about 2 – 3 passages. As cell death begins to occur, morphology and cell performance...

I am using your ProSieve™ Color Protein Markers and the molecular weights (MW) of my protein of interest is not what I expected when compared to the ProSieve™ Markers. Why is this?

The proteins in our ProSieve™ Color Markers are made of proteins that are individually prestained using colored dyes.The level of dye bound can affect the migration of the protein and this can vary slightly lot to lot. Therefore, it is important to...

What age are the rats that your Clonetics™ Rat DRGs are isolated from?

The Rat Neonatal Dorsal Root Ganglion (DRG) Neurons (R-DRG-505) are isolated from neonatal, about 2-3 days old.The Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons (R-eDRG-515) are isolated from embryonic rats (E14-15).

Will the GFAP expression in your Clonetics™ Normal Human Astrocytes remain the same throughout the culture?

The GFAP expression decreases rapidly with passaging. The results that we give are performed in the 1st passage out of cryopreservation.

What types of gels do you have for the FlashGel™System?

We offer FlashGel™ Cassettes for DNA, DNA recovery and RNA separation. FlashGel™ DNA Cassettes are available at either 1.2% or 2.2% agarose, and in 12+1 single-tier (13 wells) or 16+1 double-tier formats (34 wells). FlashGel™ DNA Recovery Cassettes...

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