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6403 results sorted by
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Export Citations
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This is an area that is up for debate. There is evidence that mycoplasma can survive in the absence of cells, and some may proliferate, but as a rule they are much happier if cells are present.
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#1-2 is to determine if the media contains Adenylate Kinase (AK), the enzyme detected in Toxilight™ Assay1. Run compound in buffer with ToxiLight™ Reagents.There should be no light output.2. Run compound/Media with ToxiLight™ Reagents.If...
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Toxilight works much better at low cell numbers, for adherent cell lines < 10,000 in one well of a 96well plate and < 50,000 for suspension.We would normally seed 10,000 cells/well when using adherent cells to allow enough room in the well for...
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HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH...
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To do the VialIght™ Plus Assay on larger volume tissue culture ( eg 6 well , 24 well) add the cell lysis reagent equal to half the volume in the plate ( ie 100 µl culture use 50 µl cell lysis). Wait 10 minutes for the lysis . Then follow the protocol...
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Magnesium (Mg) is a cofactor for both luciferase and Adenylate Kinase (AK) and will speed up their reactions, but this amount of MgCl2 wouldn't make a significant difference.
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EDTA is a chelator of metal ions like calcium and magensium. It is used with trypsin because it helps bind up the divalent cations in the medium. It helps to keep the cells from sticking to each other. If its not there….you would need a higher...
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The ViaLight™ Plus Cell Lysis Reagent inhibits ATPase activity - mainly by using a low pH and denaturing the cellular proteins. This is why the ViaLight™ Plus Assay has such a good half life. So cell expressing high ATPase will probably not...
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The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors).Transfection protocols can be found...
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ToxiLight does not need a standard curve. You can just plot the RLUs produced including an untreated control. If you want you can create a 100% lysis control by using the ToxiLight™ 100% Lysis Control Set LT07-517 or by heating a...
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