Search Results

Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME.

This review encompasses the most important advances in liver functions and hepatotoxicity and analyzes which mechanisms can be studied in vitro. In a complex architecture of nested, zonated lobules, the liver consists of approximately 80 %...

Authors: Godoy P1, Hewitt NJ, Albrecht U, Andersen ME, Ansari N, Bhattacharya S, Bode JG, Bolleyn J, Borner...

Bone marrow-derived mesenchymal stem cell-loaded fibrin patches act as a reservoir of paracrine factors in chronic myocardial infarction.

The combination of mesenchymal stem cells and tissue-engineered fibrin patches improves the therapeutic efficacy of stem cells. In vivo cardiac magnetic resonance (4.7 Tesla) and ex vivo high-spatial resolution CMR were used to track the fate of...

Authors: Blondiaux E1,2, Pidial L1, Autret G1,3, Rahmi G1, Balvay D1,3, Audureau E4, Wilhelm C5, Guerin CL1,...

Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs

New techniques able to monitor the maturation of tissue engineered constructs over time are needed for a more efficient control of developmental parameters. Here, a label-free fluorescence lifetime imaging (FLIm) approach implemented through a single...

Authors: Alfonso-Garcia A1, Shklover J1, Sherlock BE1, Panitch A1, Griffiths LG2, Marcu L1

CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells.

Immunotherapies with chimeric antigen receptor (CAR) T cells and checkpoint inhibitors (including antibodies that antagonize programmed cell death protein 1 [PD-1]) have both opened new avenues for cancer treatment, but the clinical potential of...

Authors: Rupp LJ1, Schumann K, Roybal KT, Gate RE, Ye CJ, Lim WA, Marson A.

SST Peer Reviewed

CRISPR-Cpf1 correction of muscular dystrophy mutations in human cardiomyocytes and mice.

Duchenne muscular dystrophy (DMD), caused by mutations in the X-linked dystrophin gene (DMD), is characterized by fatal degeneration of striated muscles. Dilated cardiomyopathy is one of the most common lethal features of the disease. We deployed...

Authors: Zhang Y, Long C, Li H, McAnally JR, Baskin KK1,2,3, Shelton JM4, Bassel-Duby R, Olson EN,

SST Peer Reviewed

Osteogenic commitment and differentiation of human mesenchymal stem cells by low-intensity pulsed ultrasound stimulation.

Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30?mW/cm2 LIPUS stimulation on commercially available...

Authors: Costa V1, Carina V1, Fontana S2, De Luca A1, Monteleone F2, Pagani S3, Sartori M4, Setti S5, Faldini...

Blueberry Metabolites Attenuate Lipotoxicity-Induced Endothelial Dysfunction.

SCOPE:Lipotoxicity-induced endothelial dysfunction is an important vascular complication associated with diabetes. Clinical studies support the vascular benefits of blueberry anthocyanins, but the underlying mechanism is unclear. The hypothesis that...

Authors: Bharat D1, Cavalcanti RRM1, Petersen C1, Begaye N1, Cutler BR1, Costa MMA1, Ramos RKLG1, Ferreira...

Imaging of extracellular vesicles derived from human bone marrow mesenchymal stem cells using fluorescent and magnetic labels.

Background:Mesenchymal stem cells have been shown therapeutic in various neurological disorders. Recent studies support the notion that the predominant mechanism by which MSCs act is through the release of extracellular vesicles (EVs). EVs seem to...

Authors: Dabrowska S1, Del Fattore A2, Karnas E3,4, Frontczak-Baniewicz M5, Kozlowska H6, Muraca M7, Janowski...

CRISPR/Cas9-Induced (CTG·CAG)n Repeat Instability in the Myotonic Dystrophy Type 1 Locus: Implications for Therapeutic Genome Editing.

Myotonic dystrophy type 1 (DM1) is caused by (CTG·CAG)n-repeat expansion within the DMPK gene and thought to be mediated by a toxic RNA gain of function. Current attempts to develop therapy for this disease mainly aim at destroying or blocking...

Authors: van Agtmaal EL, André LM, Willemse M, Cumming SA, van Kessel ID, van den Broek WJ, Gourdon G,...

PHACTR1 splicing isoforms and eQTLs in atherosclerosis-relevant human cells.

BACKGROUND:Genome-wide association studies (GWAS) have identified a variant (rs9349379) at the phosphatase and actin regulator 1 (PHACTR1) locus that is associated with coronary artery disease (CAD). The same variant is also an expression...

Authors: Codina-Fauteux VA, Beaudoin M, Lalonde S, Lo KS, Lettre G.

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