faq

Q:	How to determine if a compound interferes with Toxilight™ Non-Distructive Cytotoxicity BioAssay
A:	#1-2 is to determine if the media contains Adenylate Kinase (AK), the enzyme detected in Toxilight™ Assay 1. Run compound in buffer with ToxiLight™ Reagents. There should be no light output. 2. Run compound/Media with ToxiLight™ Reagents. If you get's light output then most likely there is AK present in the media or serum and AK free media/serum should be used. The liklihood of there being ATP in media/serum is extremely low to rare. #3-4 to determine if a compound interferes with the kit: Run the ATP control LT27-008 in buffer, not media, and determine if there is less signal when the compound is added this means the compound is probably interfering with the kit.

How to determine if a compound interferes with Toxilight™ Non-Distructive Cytotoxicity BioAssay

#1-2 is to determine if the media contains Adenylate Kinase (AK), the enzyme detected in Toxilight™ Assay

1. Run compound in buffer with ToxiLight™ Reagents.
There should be no light output.

2. Run compound/Media with ToxiLight™ Reagents.
If you get's light output then most likely there is AK present in the media or serum and AK free media/serum should be used. The liklihood of there being ATP in media/serum is extremely low to rare.

#3-4 to determine if a compound interferes with the kit:

Run the ATP control LT27-008 in buffer, not media, and determine if there is less signal when the compound is added this means the compound is probably interfering with the kit.

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Bioassay