faq

Q:	I am using the acute leukemia HL-60 cell line for CAR-T cells experiments. Do you have a transfection protocol for genome editing?
A:	The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors). Transfection protocols can be found here: https://knowledge.lonza.com/cell?id=64&search=hl60 You can use the optimized protocol for transfection of CRISPR/Cas9 complexes, there is no modification needed. More information about all human blood cells, functions and role in the immune response are in our new hematopoetic product page: https://bioscience.lonza.com/lonza_bs/CH/en/immuno-and-hematopoietic-knowledge-center

I am using the acute leukemia HL-60 cell line for CAR-T cells experiments. Do you have a transfection protocol for genome editing?

The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors).

Transfection protocols can be found here:

https://knowledge.lonza.com/cell?id=64&search=hl60

You can use the optimized protocol for transfection of CRISPR/Cas9 complexes, there is no modification needed.

More information about all human blood cells, functions and role in the immune response are in our new hematopoetic product page:

https://bioscience.lonza.com/lonza_bs/CH/en/immuno-and-hematopoietic-knowledge-center

Related Cells:

HL-60 ; T cell, human peripheral blood unstim. ; T cell, human stim.

Categories:

Transfection

Cell Lines and Primary Cancer Cells

Research Areas:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Stem Cells