I am using the acute leukemia HL-60 cell line for CAR-T cells experiments. Do you have a transfection protocol for genome editing?


The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors).

Transfection protocols can be found here:


You can use the optimized protocol for transfection of CRISPR/Cas9 complexes, there is no modification needed.

More information about all human blood cells, functions and role in the immune response are in our new hematopoetic product page:


Cell Lines and Primary Cancer Cells
Research Areas:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Stem Cells