What is the purpose of EDTA in trypsin? 


EDTA is needed with trypsin because it helps bind up the divalent cations in the medium.  Like calcium and magnesium.  Helps to keep the cells from sticking to each other.  If its not there….you would need a higher concentration of trypsin to get the cells to detach.  don’t want to use a PBS with calcium and magnesium to wash the cells as it may cause the same problem. 

Classical Media and Reagents
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