To do the VialIght™ Plus Assay on larger volume tissue culture ( eg 6 well , 24 well) add the cell lysis reagent equal to half the volume in the plate ( ie 100 µl culture use 50 µl cell lysis). Wait 10 minutes for the lysis . Then follow the protocol and transfer 100 µl of cell lysate into a luminescent compatible plate, add 100 µl AMR Plus, incubate for 2 minutes and then measure.