Toxilight works much better at low cell numbers, for adherent cell lines < 10,000 in one well of a 96well plate and < 50,000 for suspension.

We would normally seed 10,000 cells/well when using adherent cells to allow enough room in the well for the control untreated cells to proliferate so that the AK from these samples is not artificially high due to lack of nutrients and growth room.

If customers want to start with higher cell numbers we suggest using the sampling procedure, possibly taking 10 or even 5 ul sample to reduce the amount of adenylate kinase in the sample.