ToxiLight does not need a standard curve. You can just plot the RLUs produced including an untreated control. If you want you can create a 100% lysis control by using the ToxiLight™ 100% Lysis Control Set LT07-517 or by heating a sample of their untreated cells at 56°C for 1h.

Measuring this and then comparing it with the RLUs produced from their samples will enable them to determine percent cytoxicity if that is what they require.