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For cultivation of Smooth Muscle Cells - Can the insulin be left out of the SmGM™-2 Media?

Yes, insulin can be left out of the SmGM™-2 media for as long as 1-3 passages.Insulin is beneficial to the cells for long term growth (ie. 4 - 5 passages), however smooth muscle cells do survive quite well short term without insulin.Insulin is not a...

Where are Lonza’s Normal Human Osteoblasts (NHOst) cells isolated from?

Lonza’s Normal Human Osteoblasts (NHOst) are isolated by explant, and the tissue is from the spongy section of the bone head of the ribs, femur, or tibia.

When is the ideal time to study Lonza’s Human Knee Articular Chondrocytes (NHAC-kn) after re-differentiation?

For maximum re-differentiation and therefore maximum collagen production, Lonza’s Human Knee Articular Chondrocytes should be studied within the first passage after re-differentiation. Serial expansion in monolayer culture after re-differentiation...

How would you describe the morphology of Lonza’s Normal Human Osteoblasts (NHOst)?

Lonza’s Normal Human Osteoblasts (NHOst) generally appear fibroblastic. They are cuboidal in lower densities and become spindle shaped as they become confluent.

What is the percentage of purity of Lonza’s Normal Human Osteoblasts (NHOst) cells?

It is not easy to put an exact number to the purity of the NHOst populations because the tests used are all functional assays: alkaline phosphatase production and bone mineralization, which unless you have synchronized cultures, not all the cells are...

What microtiter plates would you recommend for running a MycoAlert® and MycoAlert® Plus Assay on a plate luminometer?

We would recommend either Porvair Sciences 204003 or Corning Costar 3912 plates to use with the MycoAlert® and MycoAlert® Plus Assay. Both of these are solid white 96-well plates and give low background and optimal results when used with the...

Are Lonza’s Normal Human Osteoblasts (NHOst) contact inhibited?

No, Lonza’s Normal Human Osteoblasts (NHOst) are not contact inhibited and tend to grow on top of each other after becoming confluent.

What buffer conditions give best resolution for agarose electrophoresis?

For small DNA fragments (<1,000 bp) when recovery is not necessary, we recommend the use of 1X TBE Buffer. Gels made with TBE Buffer give sharper bands than gels made with TAE Buffer. TBE results in better resolution for closely spaced DNA bands....

Why does Lonza recommend two different concentrations of the B-Glycerophosphate in the Normal Human Osteoblasts (NHOst) differentiation protocol?

Lonza R&D has found some NHOst cell strains to be more sensitive to B-Glycerophosphate than others. While it is necessary to differentiate, at too high of concentrations the cells will lift off dish, curl up and die. As such, Lonza recommends...

How should I cast my agarose gels to get the best resolution?

We usually cast agarose gels 3 mm to 4 mm thick. The gel volume needed can easily be estimated by measuring the surface area of the casting chamber, then multiplying by the gel thickness. Thinner gels can be cast on GelBond™ Support Film, and/or cast...

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