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Can I reuse the 100µl Nucleocuvette® Vessel?

We do not recommend reusing the conductive polymer cuvettes as performance decreases when cuvettes are used more than once.

I've had my Clonetics™ Cells in culture for a few months, they're no longer growing and don't look like healthy cells, what's wrong?

All Clonetics™ Cells are primary cells with a finite life span. Most Clonetics™ Cells are guaranteed for 10 – 15 additional population doublings, which equates to about 2 – 3 passages. As cell death begins to occur, morphology and cell performance...

How much DNA should I load per well of an agarose gel?

The amount to load per well is variable. What is most important is how much DNA there is in the bands you wish to resolve. The least amount of DNA that can be consistently detected with ethidium bromide is about 10 ng. The most DNA you can have in a...

How many cells do I have to use for each well of the 16-well Nucleocuvette® Strip?

This depends on the cell type you are working with. In our Optimized Protocols the cell numbers range from 2.5 x 10^4 cells (mouse DC) up to 1x 10^6 cells (human T cells). Detailed information is provided in our 4D-Nucleofector® Optimized Protocols.

At what voltage should I run an agarose gel?

We recommend running agarose gels at 4–10 volts/cm (cm is determined by measuring the interelectrode distance, not the gel length) under normal horizontal electrophoretic conditions.If the voltage is too high, band streaking, especially for DNA...

Do I have to apply the same program to each well of the 16-well Nucleocuvette®?

No, each well can be addressed separately. You may apply up to 16 different programs to one 16-well Nucleocuvette® Strip.

How many 100 µl Nucleocuvettes® can be transfected in parallel using the 4D-Nucleofector® System?

The 4D-Nucleofector® X Unit can work with two 100µl Nucleocuvettes® in parallel. However, the operation software allows defining up to 50 Nucleocuvettes® per experiment. These cuvettes will be transfected by the system continuously in...

What is the difference between NuSieve™ 3:1 and NuSieve™ GTG™ Agaroses?

NuSieve™ 3:1 Agarose is a standard melting temperature agarose. The resolution range for NuSieve™ 3:1 Agarose is 50 bp–1000 bp. NuSieve™ 3:1 Agarose is designed for analytical electrophoresis; its high gel strength also makes it ideal for use in...

Can I optimize the transfection efficiency or cell viability after the 4D-Nucleofector® Cell Line Optimization any further?

We offer a program fine tuning matrix for Optimization of Nucleofection® Conditions - A Short Guideline (lonza.com)Based on your current most satisfactory pulse(s) it provides guidelines which further programs to test to either increase cell...

What is a GTG™ Agarose?

GTG™ stands for Genetic Technology Grade™. GTG™ Grade Agarose is recommended for preparative DNA electrophoresis, or when further enzymatic manipulation of DNA is required. These agaroses are extensively tested to ensure maximum compatibility with...

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