Data Type
Cell Information
(860)
Citations
(4997)
FAQ
(523)
Culture Media
(61)
Category
Primary Cells and Media
(201)
Transfection
(168)
Laboratory Instrumentation
(73)
Endotoxin and Pyrogen Testing
(63)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
Classical Media and Reagents
(7)
3D Cell Culture
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(135)
Cancer Research/Cell Biology
(77)
Immunotherapy / Hematology
(61)
Gene Expression
(56)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Toxicology
(23)
Regenerative medicine
(19)
Dermatology/Tissue Engineering
(14)
Drug Discovery
(7)
Parasitology
(2)
Gastroenterology
(1)
+ Show All
523 results sorted by
relevance
alphabetical
newest first
oldest first
W
h
a
t
a
g
e
a
r
e
t
h
e
m
i
c
e
a
n
d
r
a
t
s
t
h
a
t
y
o
u
r
C
l
o
n
e
t
i
c
s
™
M
o
u
s
e
a
n
d
R
a
t
A
s
t
r
o
c
y
t
e
s
a
r
e
i
s
o
l
a
t
e
d
f
r
o
m
?
They are embryonic. The rats are E18 or E19 and the mice are E14 or E15.
M
y
s
u
s
p
e
n
s
i
o
n
-
a
d
a
p
t
e
d
H
E
K
2
9
3
(
o
r
C
H
O
)
c
e
l
l
s
t
e
n
d
t
o
c
l
u
m
p
i
n
c
u
l
t
u
r
e
.
H
o
w
c
a
n
I
a
v
o
i
d
t
h
i
s
?
Efficient protein production experiments require growth of the host cell in single-cell suspension, something that is sometimes difficult to achieve since most established cell lines retain their adherent growth characteristics. Commercially...
W
h
a
t
s
p
e
c
i
e
s
o
f
r
a
t
a
r
e
t
h
e
C
l
o
n
e
t
i
c
s
™
R
a
t
A
s
t
r
o
c
y
t
e
s
i
s
o
l
a
t
e
d
f
r
o
m
?
The rats are Sprague-Dawley.
H
o
w
c
a
n
I
i
m
p
r
o
v
e
H
u
m
a
n
H
e
p
a
t
o
c
y
t
e
v
i
a
b
i
l
i
t
y
p
o
s
t
N
u
c
l
e
o
f
e
c
t
i
o
n
™
o
n
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
d
e
v
i
c
e
?
In addition to proper cell handling and using culture media optimized for long term culturing of hepatocytes (Lonza Basal medium supplemented with BulletKit), we recommend separating the healthy hepatocytes from the dead cells and debris in the...
H
o
w
l
o
n
g
w
i
l
l
y
o
u
r
C
l
o
n
e
t
i
c
s
™
r
a
t
D
R
G
'
s
(
d
o
r
s
a
l
r
o
o
t
g
a
n
g
l
i
o
n
c
e
l
l
s
)
r
e
m
a
i
n
v
i
a
b
l
e
i
n
c
u
l
t
u
r
e
?
The cells can remain viable in culture for about 28 days.
D
o
t
h
e
C
l
o
n
e
t
i
c
s
™
R
a
t
a
n
d
M
o
u
s
e
A
s
t
r
o
c
y
t
e
s
r
e
q
u
i
r
e
a
n
e
x
t
r
a
c
e
l
l
u
l
a
r
m
a
t
r
i
x
f
o
r
p
l
a
t
i
n
g
?
No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware
W
h
a
t
i
s
t
h
e
b
e
s
t
w
a
y
t
o
c
e
n
t
r
i
f
u
g
e
H
u
m
a
n
H
e
p
a
t
o
c
y
t
e
s
p
r
i
o
r
t
o
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
In order to avoid cell compaction in the pellet and difficulties in cell resuspension, we recommend using round bottom 2 ml vials or 50 ml BD Falcon™ tubes for all centrifugation steps prior to Nucleofection™.
W
h
a
t
a
g
e
a
r
e
t
h
e
r
a
t
s
t
h
a
t
y
o
u
r
C
l
o
n
e
t
i
c
s
™
R
a
t
D
R
G
s
a
r
e
i
s
o
l
a
t
e
d
f
r
o
m
?
The Rat Neonatal Dorsal Root Ganglion (DRG) Neurons (R-DRG-505) are isolated from neonatal, about 2-3 days old.The Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons (R-eDRG-515) are isolated from embryonic rats (E14-15).
H
o
w
m
a
n
y
m
y
c
o
p
l
a
s
m
a
s
p
e
c
i
e
s
e
x
i
s
t
?
W
h
i
c
h
a
r
e
t
h
e
u
s
u
a
l
s
u
s
p
e
c
t
s
i
n
c
e
l
l
c
u
l
t
u
r
e
?
More than 180 species have been identified of which 20 distinct Mycoplasma and Acholeplasma species from human, bovine and swine have been isolated from cell culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale,...
W
h
e
n
c
a
n
I
s
t
a
r
t
i
n
d
u
c
t
i
o
n
s
t
u
d
i
e
s
o
n
H
u
m
a
n
H
e
p
a
t
o
c
y
t
e
s
t
r
a
n
s
f
e
c
t
e
d
w
i
t
h
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
?
We recommend allowing the transfected hepatocytes to recover in Plating medium for 72 hours post-Nucleofection®.For induction studies (e.g. CYP3A4), the Plating medium is replaced with maintenance medium (William's E without FCS and EGF) and...
PREVIOUS
PAGE
10
PAGE
11
PAGE
12
PAGE
13
PAGE
14
PAGE
15
PAGE
16
PAGE
17
PAGE
18
NEXT