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Are Lonzas Smooth Muscle Cells contractile or synthetic?

Lonza’s Smooth Muscle Cells are synthetic in their proliferating state. As the cells begin to differentiate, they become contractile.

Can heparin be excluded when culturing endothelial cells? What is the purpose of heparin in the endothelial cell culture media?

Heparin is added because it creates a better environment for FGF binding. Removing heparin for one passage should not have a noticeable effect, however for successive passaging, effects on proliferation may become more significant. It should be noted...

How many passages can I use the Clonetics diseased cells?

Proliferative capacity of Lonza's Clonetics™ diseased cells is tested through one or more passages (depending on the cell type) for information only.It is NOT a specification where a minimum number of doublings must be obtained in order to pass...

Do we know the severity of the asthma or the genotype of the cystic fibrosis donors?

Generally, information on the severity of asthma is not available. For our diseased cells from donors with cystic fibrosis, we usually have information on the genotype of the donor. Please contact our scientific support department for further...

Where are Lonzas Normal Human Osteoblasts (NHOst) cells isolated from?

Lonza’s Normal Human Osteoblasts (NHOst) are isolated by explant, and the tissue is from the spongy section of the bone head of the ribs, femur, or tibia.

Which isotype of VEGF is used in the EGM®-2 and EGM®-2MV Growth Media?

The isotype of VEGF is used in the EGM®-2 and EGM®-2MV Growth Media is isotype 165.

What is the concentration of sugar in Lonzas endothelial cell media?

While the complete formulation of all of Lonza’s specialty media are proprietary, the EBM™, EBM™-2, and EBM™ phenol red free basal media will all contain D-glucose (dextrose) [CAS number 50-99-7] at exactly 1.0 g/L (5.55 mM). There are no other...

What is the difference between each of the types of HUVECs that Lonza offers?

When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the cells in?Do you require pooled or single donor cells?Do you require additional...

Do I need to heat inactivate the FBS contained in the SingleQuots Kit?

No, we do not recommend heat inactivating the FBS provided in the SingleQuots™ Kit. Heat inactivating the serum can alter cell performance and denature essential components in the serum.

Is Lonza's fresh bone marrow filtered?

The fresh bone marrow is filtered only if visible clots are present. If the technical assistance notices clots in the marrow, they pour the marrow over a filter which removes the clot from the aspirate. No other manipulation of the product takes...
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