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What is the difference between NuSieve™ 3:1 and NuSieve™ GTG™ Agaroses?

NuSieve™ 3:1 Agarose is a standard melting temperature agarose. The resolution range for NuSieve™ 3:1 Agarose is 50 bp–1000 bp. NuSieve™ 3:1 Agarose is designed for analytical electrophoresis; its high gel strength also makes it ideal for use in...

Will the GFAP expression in your Clonetics™ Normal Human Astrocytes remain the same throughout the culture?

The GFAP expression decreases rapidly with passaging. The results that we give are performed in the 1st passage out of cryopreservation.

What is a GTG™ Agarose?

GTG™ stands for Genetic Technology Grade™. GTG™ Grade Agarose is recommended for preparative DNA electrophoresis, or when further enzymatic manipulation of DNA is required. These agaroses are extensively tested to ensure maximum compatibility with...

Do I need to purchase a special chamber to use Reliant™ and Latitude™ Precast Gels?

Reliant™ and Latitude™ Precast Agarose Gels are designed to run in standard horizontal electrophoresis chambers.As long as there is room on the chamber platform for the gel, the chamber should be suitable. Measure the chamber platform and check...

What medium and which cytokines are recommended for Natural Killer (NK) cell culture?

For the cultivation of Natural Killer (NK) cells we recommend using the X-VIVO 15 medium.In regard to cytokines, this varies depending upon the application, but many cited publications indicate using IL-2 for activation and longer term culture.

Are the materials in the FlashGel™ Cassette hazardous?

The stain in the FlashGel™ Cassette is present at such low levels that it is not considered hazardous according to OSHA and EU hazard criteria. A copy of the MSDS is available online. However, as the stain in the cassette is a potential mutagen, wear...

How is the B-ALI™ Media System performance tested?

The media system is performance tested with our pre-screened NHBE cells and that system is guaranteed to form ciliated, mucous-producing cells after 3 weeks of culture.

Do Lonza's NK cells proliferate in culture?

Publications indicate that IL-2 and IL-21 can stimulate NK cell proliferation in addition to using a human serum supplemented medium.

In Air-Liquid-Interface (ALI) Culture, won't the apical surface of B-ALI™ Cells dry out?

No, the cells secrete fluid at the apical side but also moisture is drawn through the cell layer from the basal layer.

I stained my gel with SYBR Green and recovered my band of interest. However, my DNA is still green. Can I remove the SYBR stain?

Yes. The SYBR stains can easily be removed from nucleic acids by ethanol precipitation. Isopropyl alcohol precipitation is somewhat less effective at removing the dye. Butyl alcohol extraction, chloroform extraction and phenol extraction do not...

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