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Most likely there was too much Nucleofector® Solution left before medium addition. Please try to aspirate solution after Nucleofection® as complete as possible without disturbing cell adherence, but add medium quickly to avoid cells getting...
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Yes. The cells do expand in culture.
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They are embryonic. The rats are E18 or E19 and the mice are E14 or E15.
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Preparation of coverslips: At Lonza we used coverslips from Menzel (part of Thermo Scientific). We think that the coverslips have such a good quality that they don’t have to be cleaned prior to use.If you want to use other coverslips or prefer...
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The rats are Sprague-Dawley.
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The cells can remain viable in culture for about 28 days.
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Yes, the AdipoRed™ Assay Reagent can be used on many other cell types that accumulate triglycerides. It may have to be optimized for specific applications depending on the lipid that is stained.
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Lonza's current portfolio for human diseased airway cells includes:- Bronchial Epithelial Cells- Lung Fibroblasts- Bronchial Smooth Muscle Cells...from asthmatic and COPD donors and if available from donors with Cystic fibrosis.An overview of...
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No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware
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The recommended media does contain glucose. Please contact Scientific Support for glucose concentrations.
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