Data Type
Cell Information
(858)
Citations
(4903)
FAQ
(515)
Culture Media
(69)
Category
Primary Cells and Media
(211)
Transfection
(164)
Laboratory Instrumentation
(70)
Endotoxin Detection
(54)
Bioassay
(43)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(31)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(7)
3D Cell Culture
(6)
Classical Media and Reagents
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(141)
Cancer Research/Cell Biology
(84)
Immunotherapy / Hematology
(63)
Gene Expression
(56)
Endotoxin Testing
(50)
Uncategorized
(45)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Regenerative medicine
(20)
Toxicology
(20)
Dermatology/Tissue Engineering
(13)
Drug Discovery
(7)
Gastroenterology
(1)
Parasitology
(1)
+ Show All
515 results sorted by
relevance
alphabetical
newest first
oldest first
D
N
A
-
p
u
r
i
t
y
a
n
d
N
u
c
l
e
o
f
e
c
t
i
o
n
®
:
C
a
n
l
o
w
A
2
6
0
:
A
2
8
0
r
a
t
i
o
s
l
e
a
d
t
o
b
o
t
h
r
e
d
u
c
e
d
t
r
a
n
s
f
e
c
t
i
o
n
e
f
f
i
c
i
e
n
c
y
a
n
d
c
e
l
l
v
i
a
b
i
l
i
t
y
?
Yes. To check the quality of your DNA we strongly recommend determining the A260:A280 ratio. It should be ideally 1.8 - 2.0 for a good DNA preparation, but least 1.6.
I
s
i
t
p
o
s
s
i
b
l
e
t
o
q
u
a
n
t
i
f
y
t
h
e
m
y
c
o
p
l
a
s
m
a
w
i
t
h
t
h
e
M
y
c
o
A
l
e
r
t
®
M
y
c
o
p
l
a
s
m
a
D
e
t
e
c
t
i
o
n
A
s
s
a
y
?
No, the MycoAlert® Mycoplasma Detection Assay (as well as the MycoAlert® PLUS Mycoplasma Detection Assay) is not quantitative; it has been designed to give a simple Yes or No answer, pertaining to the presence of Mycoplasma.
W
h
a
t
i
s
t
h
e
a
d
v
a
n
t
a
g
e
o
f
u
s
i
n
g
c
o
n
d
u
c
t
i
v
e
p
o
l
y
m
e
r
a
s
e
l
e
c
t
r
o
d
e
m
a
t
e
r
i
a
l
f
o
r
t
h
e
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
®
X
-
U
n
i
t
a
n
d
9
6
-
w
e
l
l
U
n
i
t
?
With conductive polymer electrodes the release of metal ions into the cell suspension is avoided. Conductive polymer electrode Nucloecuvette® Vessels are used in the 4D-Nucleofector® X-Unit and 96-well Unit?
C
a
n
n
i
c
k
e
d
D
N
A
l
e
a
d
t
o
r
e
d
u
c
e
d
t
r
a
n
s
f
e
c
t
i
o
n
e
f
f
i
c
i
e
n
c
y
i
n
N
u
c
l
e
o
f
e
c
t
i
o
n
®
e
x
p
e
r
i
m
e
n
t
s
?
Yes. You should verify the integrity of your DNA on an agarose gel to see if it is degraded. Compare undigested plasmid to plasmid DNA digested with a suitable single cut restriction enzyme to linearize. Supercoiled plasmid will run faster than...
W
h
e
n
p
e
r
f
o
r
m
i
n
g
t
h
e
M
y
c
o
A
l
e
r
t
®
M
y
c
o
p
l
a
s
m
a
D
e
t
e
c
t
i
o
n
A
s
s
a
y
,
w
h
y
i
s
i
t
n
e
c
e
s
s
a
r
y
t
o
s
p
i
n
o
u
t
t
h
e
c
e
l
l
s
?
It is highly recommended that the samples are spun down prior to testing. If cells are present in the assay they will contribute to the initial reading producing a higher background. This in turn can decrease the difference between the background and...
C
a
n
I
u
s
e
t
h
e
s
e
t
t
i
n
g
s
r
e
c
o
m
m
e
n
d
e
d
f
o
r
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
I
/
I
I
o
r
2
b
S
y
s
t
e
m
w
i
t
h
m
y
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
®
S
y
s
t
e
m
?
No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...
W
h
a
t
p
r
o
t
o
c
o
l
s
h
o
u
l
d
I
u
s
e
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
o
f
p
a
t
i
e
n
t
d
e
r
i
v
e
d
b
l
o
o
d
s
a
m
p
l
e
s
,
e
.
g
.
l
e
u
k
e
m
i
a
o
r
l
y
m
p
h
o
m
a
c
e
l
l
s
?
Unfortunately, we do not have a ready-to-use protocol for Chronic lymphocytic leukemia (CLL) or other blood cell derived cancer cells. The cells often have a chromosomal aberration and show the properties of a cell line. Therefore we suggest to...
C
a
n
I
r
e
u
s
e
t
h
e
1
0
0
µ
l
N
u
c
l
e
o
c
u
v
e
t
t
e
®
V
e
s
s
e
l
?
We do not recommend reusing the conductive polymer cuvettes as performance decreases when cuvettes are used more than once.
H
o
w
l
o
n
g
i
s
t
h
e
M
y
c
o
A
l
e
r
t
®
M
y
c
o
p
l
a
s
m
a
D
e
t
e
c
t
i
o
n
A
s
s
a
y
s
i
g
n
a
l
s
t
a
b
l
e
?
Please follow the protocol exactly as written – add reagent, wait 5 min and read, add substrate, wait 10 minutes and read again.
H
o
w
m
a
n
y
c
e
l
l
s
d
o
I
h
a
v
e
t
o
u
s
e
f
o
r
e
a
c
h
w
e
l
l
o
f
t
h
e
1
6
-
w
e
l
l
N
u
c
l
e
o
c
u
v
e
t
t
e
®
S
t
r
i
p
?
This depends on the cell type you are working with. In our Optimized Protocols the cell numbers range from 2.5 x 10^4 cells (mouse DC) up to 1x 10^6 cells (human T cells). Detailed information is provided in our 4D-Nucleofector® Optimized Protocols.
PREVIOUS
PAGE
10
PAGE
11
PAGE
12
PAGE
13
PAGE
14
PAGE
15
PAGE
16
PAGE
17
PAGE
18
NEXT