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515 results sorted by
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A
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S
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The 4D-Nucleofector® Unit was tested by TÜV Rheinland Product Safety GmbH and found to be in compliance with the following standards:• IEC 61010-1The 4D-Nucleofector® Unit was tested by TÜV Rheinland of North America Inc. and found to be in...
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Yes. In addition to the tailor-made MycoAlert® Mode it also has a "Single Read" mode which is suited for unprocessed luminescence readings, e.g. ATP-based cell proliferation or cytotoxicity assays, or Luciferase reporter gene assays.
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Cell death will usually be observed during the first few days of growth, resulting in cell debris in the culture – this is normal. Cell cultivation should be continued and surviving cells should start to develop. By day 4, neurite networks will be...
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Yes, this is a routine observation.Fluorescent fusion proteins can appear less bright than the fluorescent protein itself. This may be due to differences in protein folding of the fused protein.
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For optimal results, poly-D-lysine with laminin should be used, especially for the hippocampus cells.Poly-D-lysine without the laminin or poly-L-lysine could also be used for the rat and mouse neuronal cells.
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The cells are pooled from whole litters, including both males and females.
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It is very critical. Waiting too long for analysis post transfection can lead to loss of peak expression. The optimal time for analysis is related to two factors: stability of protein being expressed and transfection method. For a short-lived protein...
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Both are of embryonic origin: the rats are E18 or E19 and the mice are E14 or E15.
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We don't sell the individual components of the kits separately because our product line is a kit concept line to ensure the use of certified products with quality controlled lot numbers that guarantee optimal results.
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The rats are Sprague-Dawley and the mice are CD1 or C57.
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