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515 results sorted by
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No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...
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Preparation of coverslips: At Lonza we used coverslips from Menzel (part of Thermo Scientific). We think that the coverslips have such a good quality that they don’t have to be cleaned prior to use.If you want to use other coverslips or prefer...
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We do not recommend reusing the conductive polymer cuvettes as performance decreases when cuvettes are used more than once.
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This depends on the cell type you are working with. In our Optimized Protocols the cell numbers range from 2.5 x 10^4 cells (mouse DC) up to 1x 10^6 cells (human T cells). Detailed information is provided in our 4D-Nucleofector® Optimized Protocols.
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No, each well can be addressed separately. You may apply up to 16 different programs to one 16-well Nucleocuvette® Strip.
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Yes, the AdipoRed™ Assay Reagent can be used on many other cell types that accumulate triglycerides. It may have to be optimized for specific applications depending on the lipid that is stained.
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The Human Skeletal Muscle Myoblasts are sourced from normal post-gestational tissue of cadavers from 1 day to 40 years old. The HSMMs are isolated from the quadriceps or psoas muscle.
H
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The 4D-Nucleofector® X Unit can work with two 100µl Nucleocuvettes® in parallel. However, the operation software allows defining up to 50 Nucleocuvettes® per experiment. These cuvettes will be transfected by the system continuously in...
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The recommended media does contain glucose. Please contact Scientific Support for glucose concentrations.
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Human Skeletal Muscle Myoblasts usually begin to form multinucleated myotubes when the culture reaches 50-70% confluence. Myotubes are formed by the fusion of several to many myoblasts. Addition of differentiation medium, when the culture reaches...
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