Data Type
Cell Information
(858)
Citations
(4968)
FAQ
(515)
Culture Media
(61)
Category
Primary Cells and Media
(200)
Transfection
(170)
Laboratory Instrumentation
(73)
Endotoxin Detection
(54)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
3D Cell Culture
(6)
Classical Media and Reagents
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(135)
Cancer Research/Cell Biology
(77)
Immunotherapy / Hematology
(63)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Toxicology
(22)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(14)
Drug Discovery
(7)
Parasitology
(2)
Gastroenterology
(1)
+ Show All
515 results sorted by
relevance
alphabetical
newest first
oldest first
C
a
n
I
i
n
t
e
g
r
a
t
e
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
D
e
v
i
c
e
i
n
t
o
a
l
i
q
u
i
d
h
a
n
d
l
i
n
g
s
y
s
t
e
m
?
Yes. The 96-well Shuttle® Device is designed in such a way that it is addressable by robot grippers. Furthermore, the software contains the required interface to be connected with the LHS software. However, the practical implementation for each...
H
o
w
l
o
n
g
d
o
e
s
i
t
t
a
k
e
t
o
o
b
t
a
i
n
s
t
a
b
l
e
t
r
a
n
s
f
e
c
t
a
n
t
s
?
Depending on individual cell type and doubling rate, selection of stable transfectants will take between 7 and 28 days. Expansion and characterization of single cell clones will take several weeks in addition.
A
c
c
o
r
d
i
n
g
t
o
w
h
i
c
h
s
t
a
n
d
a
r
d
i
s
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
S
y
s
t
e
m
c
e
r
t
i
f
i
e
d
?
The 4D-Nucleofector® Unit was tested by TÜV Rheinland Product Safety GmbH and found to be in compliance with the following standards:• IEC 61010-1The 4D-Nucleofector® Unit was tested by TÜV Rheinland of North America Inc. and found to be in...
I
n
s
t
a
b
l
e
c
e
l
l
l
i
n
e
g
e
n
e
r
a
t
i
o
n
,
I
h
a
v
e
a
v
e
r
y
h
i
g
h
t
r
a
n
s
f
e
c
t
i
o
n
e
f
f
i
c
i
e
n
c
y
,
b
u
t
m
o
s
t
o
f
m
y
c
e
l
l
s
d
i
e
d
u
r
i
n
g
s
e
l
e
c
t
i
o
n
.
I
s
t
h
i
s
t
o
b
e
e
x
p
e
c
t
e
d
?
This is the normal pattern you should expect to see. Only a small proportion (1/10,000 to 1/100) of all transfected cells will integrate the transfected DNA into their genome and become stable transfectants. The remaining cells lose the transfected...
I
s
i
t
t
y
p
i
c
a
l
f
o
r
t
h
e
f
l
u
o
r
e
s
c
e
n
c
e
o
b
s
e
r
v
e
d
f
r
o
m
m
y
t
a
g
g
e
d
p
r
o
t
e
i
n
t
o
a
p
p
e
a
r
l
e
s
s
t
h
a
n
t
h
e
e
m
p
t
y
f
l
u
o
r
e
s
c
e
n
t
v
e
c
t
o
r
?
Yes, this is a routine observation.Fluorescent fusion proteins can appear less bright than the fluorescent protein itself. This may be due to differences in protein folding of the fused protein.
C
a
n
I
u
s
e
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
2
b
S
o
l
u
t
i
o
n
s
a
n
d
O
p
t
i
m
i
z
e
d
P
r
o
t
o
c
o
l
s
w
i
t
h
t
h
e
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
®
X
-
U
n
i
t
o
r
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
S
y
s
t
e
m
?
No. The 4D-Nucleofector and 96-well Shuttle® System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle® Solutions are available for primary cells. For cell lines, three...
C
a
n
I
u
s
e
a
N
u
c
l
e
o
f
e
c
t
o
r
®
I
o
r
N
u
c
l
e
o
f
e
c
t
o
r
®
2
b
D
e
v
i
c
e
t
o
g
e
t
h
e
r
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
D
e
v
i
c
e
?
No, the 96-well Shuttle® only works in conjunction with the Nucleofector® IIs Device or our 4D-Nucleofector® Device.
I
n
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
®
E
x
p
e
r
i
m
e
n
t
,
h
o
w
c
r
i
t
i
c
a
l
i
s
t
h
e
t
i
m
e
p
o
i
n
t
f
o
r
a
n
a
l
y
s
i
s
p
o
s
t
t
r
a
n
s
f
e
c
t
i
o
n
?
It is very critical. Waiting too long for analysis post transfection can lead to loss of peak expression. The optimal time for analysis is related to two factors: stability of protein being expressed and transfection method. For a short-lived protein...
W
h
a
t
s
e
l
e
c
t
i
o
n
m
a
r
k
e
r
s
c
a
n
I
u
s
e
f
o
r
t
h
e
g
e
n
e
r
a
t
i
o
n
o
f
s
t
a
b
l
e
c
e
l
l
l
i
n
e
s
?
The most commonly used marker is the neomycin phosphotransferase (neo) gene that confers resistance to G418 to eukaryotic cells. Other markers are Puromycin, Hygromycin, Zeocin, or the HPRT gene that can be used in HPRT-deficient cells.
A
t
w
h
i
c
h
t
i
m
e
p
o
i
n
t
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
s
h
o
u
l
d
I
s
t
a
r
t
s
e
l
e
c
t
i
o
n
o
f
s
t
a
b
l
e
i
n
t
e
g
r
a
t
i
o
n
?
Antibiotics for selection should be added 24-48 h after Nucleofection®.
PREVIOUS
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
PAGE
12
PAGE
13
PAGE
14
PAGE
15
NEXT