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Can I use the Nucleofector® 2b Solutions and Optimized Protocols with the 4D-Nucleofector® X-Unit or the 96-well Shuttle® Device?

No. The 4D-Nucleofector and 96-well Shuttle® System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle® Solutions are available for primary cells. For cell lines, three...

Can I use a Nucleofector® I or Nucleofector® 2b Device together with the 96-well Shuttle® Device?

No, the 96-well Shuttle® only works in conjunction with the Nucleofector® IIs Device or our 4D-Nucleofector® Device.

For Lonza's animal neurons, how many cortical neurons can I expect from a single pup?

According to our manufacturer, you can expect 12 million cortical neurons from an average E18-E19 rat pup.You can expect 4 million cortical neurons from an average mouse pup.

What is the advantage of the Nucleofector® Technology over common electroporation (systems)?

High cell viability and high transfection efficiency with the Nucleofection® Technology are the most prominent features. Nucleofection®, i.e., the transfer of DNA into the nucleus and not only the cytosol, is essential when working with...

Is it possible to pre-plate substrates when using the 96-well Shuttle® for Nucleofection®?

Yes, pre-plating of substrates like siRNA and subsequent storage at -20°C is possible.

What does the message "weak" mean on the display of the Nucleofector® IIN, IIS or 2b after Nucleofection®?

This message means that the system cut off the pulse before it was completed (usually this is because the conductivity of the solution is at the low end of the normal range). The pulse was applied; it is just going to be a little weaker than...

What is the molecular weight (MW) of your control plasmid pmaxGFP?

The molecular weight is approximately 2.3x10e6 g/mol based on the average MW of a base pair being 660 g/mol.

Where can I find the meaning of the 4D-Nucleofector® System Error Codes?

In the back of the respective manuals, there is a chart giving a brief description of the error codes. If more information is needed or an error code is recurrent, it is best to contact Scientific Support for advice to determine if there is a...


According to which standard is the Nucleofector® System certified?

The 4D-Nucleofector® Unit was tested by TÜV Rheinland Product Safety GmbH and found to be in compliance with the following standards:• IEC 61010-1The 4D-Nucleofector® Unit was tested by TÜV Rheinland of North America Inc. and found to be in...
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