cells

Also called HEK293. Embryonic kidney cells transformed by adenovirus type 5

Cell Type:

Kidney Epith.

Tissue Origin:

kidney

Species:

human

Research Area:

Cancer Research/Cell Biology

Dermatology/Tissue Engineering

Cell Characteristics:

Adherent

Recommended Media

Pro293

Pro293 Chemically Defined, Serum-free Media were optimized to support high-density growth and recombinant protein production in 293 human kidney cells. They are chemically defined to ease regulatory compliance and downstream protein purification. They contain very low levels of recombinant human insulin, and are free of animal-origin components.

Pro293s Medium - for 293 cells growing in suspension culture
Pro293a Medium - for 293 cells growing in adherent culture

Storage = 2°C to 8°C

12-765 - Pro293s Medium (without L-glutamine or phenol red, with 0.1% Pluronic F-68)

12-764 - Pro293a Medium (without L-glutamine or phenol red, with 0.1% Pluronic F-68)
BE02-025Q - Pro293a Medium (without L-glutamine, galaktose or phenol red, with 0.1% Pluronic F-68)

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	A-023	1e6	36% ±12		Plasmid (general)	1 µg	100 µl	I/II/2b
V	A-023	1e6	57% ±16		Plasmid (general)	1 µg	100 µl	I/II/2b
SF	CM-130	2e5	92.7% ±1	88.7% ±5	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	CM-130	1e6	88.6% ±5	72.6% ±5	Plasmid (general)	2 µg	100 µl	4D X-Unit
V	Q-001	1e6	81% ±5		Plasmid (general)	5 µg	100 µl	I/II/2b
V	Q-001	1e6	84% ±1		Plasmid (general)	5 µg	100 µl	I/II/2b
SF	96-CM-130	2e5	83% ±9	93% ±4	Plasmid (general)	0.4 µg	20 µl	96-well

Citations (45)

Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair

Categories:

Transfection

Authors:

Schubert MS, Thommandru B, Woodley J, Turk R, Yan S, Kurgan G, McNeill MS, Rettig GR.

In:

Scientific Reports (2021) 11(1): 19482

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

Categories:

Transfection

Authors:

Wienert B, Nguyen DN, Guenther A, Feng SJ, Locke MN, Wyman SK, Shin J, Kazane KR, Gregory GL, Carter MAM, Wright F, Conklin BR, Marson A, Richardson CD, Corn JE

In:

Nat Commun. (2020) 11(1): 2109

Large-scale GMP-compliant CRISPR-Cas9–mediated deletion of the glucocorticoid receptor in multivirus-specific T cells

Categories:

Transfection

Authors:

Basar R, Daher M, Uprety N, Gokdemir E, Alsuliman A, Ensley E, Ozcan G, Mendt M, Hernandez Sanabria M, Kerbauy LN, Nunez Cortes AK, Li L, Banerjee PP, Muniz-Feliciano L, Acharya S, Fowlkes NW, Lu J, Li S, Mielke S, Kaplan M, Nandivada V, Bdaiwi M, Kontoyiannis AD, Li Y, Liu E, Ang S, Marin D, Brunetti L, Gundry MC, Turk R, Schubert MS, Rettig GR, McNeill MS, Kurgan G, Behlke MA, Champlin R, Shpall EJ, Rezvani K.

In:

Blood (2020) 4(14): 3357-3367

A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

Vakulskas CA, Dever DP, Rettig GR, Turk R, Jacobi AM, Collingwood MA, Bode NM, McNeill MS, Yan S, Camarena J, Lee CM, Park SH, Wiebking V, Bak RO, Gomez-Ospina N, Pavel-Dinu M, Sun W6, Bao G, Porteus MH, Behlke MA

In:

Nat Med (2018) 24(8): 1216-1224

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.

Categories:

Transfection

Authors:

Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE.

In:

Nat Biotechnol (2016) 1(1): 1-7

Cytotoxic Effects during Knock Out of Multiple Porcine Endogenous Retrovirus (PERV) Sequences in the Pig Genome by Zinc Finger Nucleases (ZFN)

Categories:

Transfection

Authors:

Semaan M, Ivanusic D, Denner J.

In:

PLoS ONE (2015) 10(4): 1-18

Genome engineering using the CRISPR-Cas9 system

Categories:

Transfection

Authors:

Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F

In:

Nat Protocols (2013) 8(11): 2281–2308

Myeloblastic cell lines mimic some but not all aspects of human cytomegalovirus experimental latency defined in primary CD34+ cell populations.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Albright ER1, Kalejta RF

In:

J Virol (2013) 87(17): 9802-12

Regulation of cell shape and adhesion by CD34.

Categories:

Primary Cells and Media

Authors:

Ohnishi H, Sasaki H, Nakamura Y, Kato S, Ando K, Narimatsu H, Tachibana K

In:

Cell Adh Migr (2013) 7(5): 426-33

Aftiphilin and gamma-Synergin are Required for Secretagogue-sensitivity of Weibel-Palade Bodies in Endothelial Cells

Categories:

Transfection

Authors:

Lui-Roberts WW, Ferraro F, Nightingale TD, Cutler DF

In:

Mol Biol Cell (2008) epub: online

Differences in the length of the carboxyl terminus mediate functional properties of neurokinin-1 receptor

Categories:

Transfection

Authors:

Lai JP, Lai S, Tuluc F, Tansky MF, Kilpatrick LE, Leeman SE, Douglas SD

In:

Proc Natl Acad Sci USA (2008) 105(34): 12605-10

The ubiquitin-like protein Plic-1 enhances the membrane insertion of GABAA receptors by increasing their stability within the endoplasmic reticulum

Categories:

Transfection

Authors:

Saliba RS, Pangalos M, Moss SJ

In:

J Biol Chem (2008) 283(27): 18538-44

Store-dependent and -independent modes regulating CRAC channel activity of human Orai1 and Orai3

Categories:

Transfection

Authors:

Zhang SL, Kozak JA, Jiang W, Yeromin AV, Chen J, Yu Y, Penna A, Shen W, Chi V, Cahalan MD

In:

J Biol Chem (2008) 283(25): 17662-71

Bad plays a more significant role than Bid and Bim in mediating cell death signals in batch cultures of HEK 293 cells

Categories:

Transfection

Authors:

Padmanabhan A, Liu S, Song Z

In:

Biotechnol Lett (2008) 30(5): 819-27

Inverse Agonist and Neutral Antagonist Actions of Antidepressants at Recombinant and Native 5-HT2C Receptors: Differential Modulation of Cell Surface Expression and Signal Transduction

Categories:

Transfection

Authors:

Chanrion B, Mannoury la Cour C, Gavarini S, Seimandi M, Vincent L, Pujol JF, Bockaert J, Marin P, Millan MJ

In:

Mol Pharmacol (2008) 73(3): 748-57

Both Orai1 and Orai3 are essential components of the arachidonate-regulated Ca2+-selective ARC channels

Categories:

Transfection

Authors:

Mignen O, Thompson JL, Shuttleworth TJ

In:

J Physiol (2008) 586(1): 185-95

Recombinant interleukin-24 lacks apoptosis-inducing properties in melanoma cells

Categories:

Transfection

Authors:

Kreis S, Philippidou D, Margue C, Rolvering C, Haan C, Dumoutier L, Renauld JC, Behrmann I

In:

PLoS ONE (2007) 2(12): e1300

Compound Heterozygosity for Mutations in LMNA in a Patient with a Myopathic and Lipodystrophic Mandibuloacral Dysplasia Type A Phenotype

Categories:

Transfection

Authors:

Lombardi F, Gullotta F, Columbaro M, Filareto A, D'Adamo M, Vielle A, Guglielmi V, Nardone AM, Azzolini V, Grosso E, Lattanzi G, D'Apice MR, Masala S, Maraldi NM, Sbraccia P, Novelli G

In:

J Clin Endocrinol Metab (2007) 92(11): 4467-71

Requirements for cellular co-trafficking of factor VIII and von Willebrand factor to Weibel-Palade bodies

Categories:

Transfection

Authors:

van den Biggelaar M, Bierings R, Storm G, Voorberg J, Mertens K

In:

J Thromb Haemost (2007) 5(11): 2235-42

In Vivo and in Vitro Intragenomic Rearrangement of TT Viruses

Categories:

Transfection

Authors:

Leppik L, Gunst K, Lehtinen M, Dillner J, Streker K, de Villiers EM

In:

J Virol (2007) 81(17): 9346-56

Endoplasmic reticulum retention of the gamma-secretase complex component Pen2 by Rer1

Categories:

Transfection

Authors:

Kaether C, Scheuermann J, Fassler M, Zilow S, Shirotani K, Valkova C, Novak B, Kacmar S, Steiner H, Haass C

In:

EMBO Rep (2007) 8(8): 743-748

A hidden oncogenic positive feedback loop caused by crosstalk between Wnt and ERK Pathways

Categories:

Transfection

Authors:

Kim D, Rath O, Kolch W, Cho KH

In:

Oncogene (2007) 26(31): 4571-9

The proline-Rich Akt substrate of 40 kDa (PRAS40) is a physiological substrate of mTOR complex 1

Categories:

Transfection

Authors:

Oshiro N, Takahashi R, Yoshino KI, Tanimura K, Nakashima A, Eguchi S, Miyamoto T, Hara K, Takehana K, Avruch J, Kikkawa U, Yonezawa K

In:

J Biol Chem (2007) 282(28): 20329-39

Specificity of olfactory receptor interactions with other G protein-coupled receptors

Categories:

Transfection

Authors:

Bush CF, Jones SV, Lyle AN, Minneman KP, Ressler KJ, Hall RA

In:

J Biol Chem (2007) 282(26): 19042-51

Expression and Regulation of the Metalloproteinase ADAM-8 during Human Neutrophil Pathophysiological Activation and Its Catalytic Activity on L-Selectin Shedding

Categories:

Transfection

Authors:

Gomez-Gaviro M, Dominguez-Luis M, Canchado J, Calafat J, Janssen H, Lara-Pezzi E, Fourie A, Tugores A, Valenzuela-Fernandez A, Mollinedo F, Sanchez-Madrid F, DÜaz-Gonzalez F

In:

J Immunol (2007) 178(12): 8053-63

Regulation of pri-microRNA BIC transcription and processing in Burkitt lymphoma

Categories:

Transfection

Authors:

Kluiver J, van den Berg A, de Jong D, Blokzijl T, Harms G, Bouwman E, Jacobs S, Poppema S, Kroesen BJ

In:

Oncogene (2007) 26(26): 3769-76

Physical interaction between the serotonin transporter and neuronal nitric oxide synthase underlies reciprocal modulation of their activity

Categories:

Transfection

Authors:

Chanrion B, Mannoury la Cour C, Bertaso F, Lerner-Natoli M, Freissmuth M, Millan MJ, Bockaert J, Marin P

In:

Proc Natl Acad Sci USA (2007) 104(19): 8119-24

STIM1 regulates Ca2+ entry via arachidonate-regulated Ca2+-selective (ARC) channels without store-depletion or translocation to the plasma membrane

Categories:

Transfection

Authors:

Mignen O, Thompson JL, Shuttleworth TJ

In:

J Physiol (2007) 579(Pt 3): 703-15

Ca2+-activated IK1 channels associate with lipid rafts upon cell swelling and mediate volume recovery

Categories:

Transfection

Authors:

Barfod ET, Moore AL, Roe MW, Lidofsky SD

In:

J Biol Chem (2007) 282(12): 8984-93

Alpha-2C-adrenergic receptors exhibit enhanced surface expression and signaling upon association with beta-2-adrenergic receptors

Categories:

Transfection

Authors:

Prinster SC, Holmqvist TG, Hall RA

In:

J Pharmacol Exp Ther (2006) 318(3): 974-81

The mTOR pathway regulates mitochondrial oxygen consumption and oxidative capacity

Categories:

Transfection

Authors:

Schieke SM, Phillips D, McCoy JP Jr, Aponte AM, Shen RF, Balaban RS, Finkel T

In:

J Biol Chem (2006) 281(37): 27643-52

Mutagenesis and molecular modeling reveal the importance of the 5-HT3 receptor F-loop

Categories:

Transfection

Authors:

Thompson AJ, Padgett CL, Lummis SC

In:

J Biol Chem (2006) 281(24): 16576-82

P-selectin binds to the D'-D3 domains of von Willebrand factor in Weibel-Palade bodies

Categories:

Transfection

Authors:

Michaux G, Pullen TJ, Haberichter SL and Cutler DF

In:

Blood (2006) 107(10): 3922-4

Apolipoprotein A-I (APO A-I) activates CDC42 signaling through ABCA1 transporter

Categories:

Transfection

Authors:

Nofer JR, Remaley AT, Feuerborn R, Wolinska I, Engel T, von Eckardstein A and Assmann G

In:

J Lipid Res (2006) 47(4): 794-803

Infection with Plasmodium berghei Boosts Antibody Responses Primed by a DNA Vaccine Encoding Gametocyte Antigen Pbs48/45

Categories:

Transfection

Authors:

Haddad D, Maciel J, Kumar N

In:

Infect Immun (2006) 74(4): 2043-51

The physiological function of von Willebrand's factor depends on its tubular storage in endothelial Weibel-Palade bodies

Categories:

Transfection

Authors:

Michaux G, Abbitt KB, Collinson LM, Haberichter SL, Norman KE, Cutler DF

In:

Dev Cell (2006) 10(2): 223-32

An AP-1/clathrin coat plays a novel and essential role in forming the Weibel-Palade bodies of endothelial cells

Categories:

Transfection

Authors:

Lui-Roberts WW, Collinson LM, Hewlett LJ, Michaux G and Cutler DF

In:

J Cell Biol (2005) 170(4): 627-636

The low density lipoprotein receptor-related protein (LRP) is a novel beta-secretase (BACE1) substrate

Categories:

Transfection

Authors:

von Arnim CA, Kinoshita A, Peltan ID, Tangredi MM, Herl L, Lee BM, Spoelgen R, Hshieh TT, Ranganathan S, Battey FD, Liu CX, Bacskai BJ, Sever S, Irizarry MC, Strickland DK and Hyman BT

In:

J Biol Chem (2005) 280(18): 17777-17785

Exocytosis of CTLA-4 Is Dependent on Phospholipase D and ADP Ribosylation Factor-1 and Stimulated during Activation of Regulatory T Cells

Categories:

Transfection

Authors:

Mead KI, Zheng Y, Manzotti CN, Perry LC, Liu MK, Burke F, Powner DJ, Wakelam MJ and Sansom DM

In:

J Immunol (2005) 174(8): 4803-4811

c-Jun NH2-terminal kinase-interacting protein-3 facilitates phosphorylation and controls localization of amyloid-beta precursor protein

Categories:

Transfection

Authors:

Muresan Z and Muresan V

In:

J Neurosci (2005) 25(15): 3741-3751

Apolipoprotein E is required for cell proliferation and survival in ovarian cancer

Categories:

Transfection

Authors:

Chen YC, Pohl G, Wang TL, Morin PJ, Risberg B, Kristensen GB, Yu A, Davidson B and Shih IeM

In:

Cancer Res (2005) 65(1): 331-337

Transient overexpression of mitochondrial transcription factor A (TFAM) is sufficient to stimulate mitochondrial DNA transcription, but not sufficient to increase mtDNA copy number in cultured cells

Categories:

Transfection

Authors:

Maniura-Weber K, Goffart S, Garstka HL, Montoya J and Wiesner RJ

In:

Nucleic Acids Res (2004) 32(20): 6015-6027

New non-viral method for gene transfer into primary cells

Categories:

Transfection

Authors:

Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K

In:

Methods (2004) 33(2): 151-163

Targeted deletion of BMK1/ERK5 in adult mice perturbs vascular integrity and leads to endothelial failure

Categories:

Transfection

Authors:

Hayashi M, Kim SW, Imanaka-Yoshida K, Yoshida T, Abel ED, Eliceiri B, Yang Y, Ulevitch RJ and Lee JD

In:

J Clin Invest (2004) 113(8): 1138-48

Extracellular Nef protein targets CD4+ T cells for apoptosis by interacting with CXCR4 surface receptors.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

James CO, Huang MB, Khan M, Garcia-Barrio M, Powell MD, Bond VC

In:

J Virol (2004) 78(6): 3099-109

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