Regulation of cell shape and adhesion by CD34.

Ohnishi H, Sasaki H, Nakamura Y, Kato S, Ando K, Narimatsu H, Tachibana K
Source: Cell Adh Migr
Publication Date: (2013)
Issue: 7(5): 426-33
Research Area:
Immunotherapy / Hematology
Basic Research
Cells used in publication:
Species: human
Tissue Origin: kidney
CD34+ cell, human
Species: human
Tissue Origin: blood
Species: human
Tissue Origin: bone marrow
Mononuclear, cord blood, human
Species: human
Tissue Origin: blood
Customer was using bone marrow derived CD34+ cells obtained from Lonza along with KG-1, HEK293T, and cord blood CD34+ cells that they isolated themselves to exhibit that expression of CD34 alone inhibits integrin-mediated cell adhesion directly or indirectly via formation and/or maintenance of a spherical cell shape and cell surface microvilli. Lonza CD34+ cells were used as a control to compare against HEK293T cells transfected (using Lipofectamine 200) with the pCpuroCMVS expression vector.
We previously reported that expression of CD43/leukosialin induces cell rounding and microvillus formation via inhibition of cell adhesion. Here, we found that CD34, a cell surface sialomucin and marker for hematopoietic progenitor cells, also inhibited cell adhesion and induced cell rounding and microvillus formation. Forced expression of CD34-induced cell rounding, microvillus formation, and phosphorylation of ezrin/radixin/moesin (ERM) proteins in HEK293T cells, while inhibiting integrin-mediated cell re-attachment. Furthermore, CD34+ blood cells and KG-1 cells, which express endogenous CD34 on their surface, were spherical in shape, surrounded by microvilli, and non-adherent to substrata. In addition, cleavage of O-sialomucin augmented integrin-mediated cell adhesion of KG-1 cells. These results suggest the involvement of CD34 in the inhibition of integrin-mediated cell adhesion and formation of the cell surface structure. The inhibitory function of CD34 in cell adhesion may affect cell shape organization via phosphorylation of ERM proteins. Cellular structures such as the spherical shape and microvilli of CD34+ cells may also contribute to regulation of cell adhesion.