KG-1
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acute myelogenous leukemia (AML) cell line
Cell Type:
Myeloid (unspec.)
Tissue Origin:
bone marrow
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Plasmid (general)
Any Platform
4D-Nucleofector® X-Unit
Nucleofector® I/II/2b
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
FF-100
1e6
63-69%
45-53%
Plasmid (general)
5 µg
100 µl
4D X-Unit
SF
FF-100
2e5
58-62%
47-55%
Plasmid (general)
1 µg
20 µl
4D X-Unit
R
V-001
2e6
68-72%
77-91%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Myeloblastic cell lines mimic some but not all aspects of human cytomegalovirus experimental latency defined in primary CD34+ cell populations.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media
Authors:
Albright ER1, Kalejta RF
In:
J Virol (2013) 87(17): 9802-12
Regulation of cell shape and adhesion by CD34.
Categories:
Primary Cells and Media
Authors:
Ohnishi H, Sasaki H, Nakamura Y, Kato S, Ando K, Narimatsu H, Tachibana K
In:
Cell Adh Migr (2013) 7(5): 426-33
Virally induced changes in cellular microRNAs maintain latency of human cytomegalovirus in CD34? progenitors.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media
Authors:
Poole E, McGregor Dallas SR, Colston J, Joseph RS, Sinclair J
In:
J Gen Virol (2011) 92(7): 1539-49
Lipid raft adhesion receptors and Syk regulate selectin-dependent rolling under flow conditions
Categories:
Transfection
Authors:
Abbal C, Lambelet M, Bertaggia D, Gerbex C, Martinez M, Arcaro A, Schapira M, Spertini O
In:
Blood (2006) 108(10): 3352-9